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C0775 Sigma

Crystal Violet

certified by the Biological Stain Commission

Synonym: Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B

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Properties

Related Categories Antibiotics, Antibiotics A to Z, Antibiotics A-F, Antifungal, Biochemicals and Reagents,
grade   certified by the Biological Stain Commission
InChI Key   ZXJXZNDDNMQXFV-UHFFFAOYSA-M
form   powder
composition   Dye content, ≥90%
mp   205 °C (dec.)(lit.)
solubility   H2O: soluble1 mg/mL

Description

Packaging

25, 100 g in glass bottle

Application

Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria.
It has been used to check cell viability. It has also been used for the staining of cells to study cell migration and invasion.

Biochem/physiol Actions

Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal violet is also used for the staining of bacteria in gram staining technique. It is also used for the staining of plant chromosomes. Crystal Violet also helps in colorimetric measurement of cell viability.

Suitability

Certified for use with the Gram stain for bacteria, Flemming triple stain with iodine for chromosomes, Lieb′s amyloid stain and Newton′s crystal violet-iodine technique for chromatin and nucleoli.

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Safety & Documentation

Safety Information

Signal word 
Danger
Hazard statements 
RIDADR 
UN 3077 9 / PGIII
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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