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C0794 Sigma

Chitosanase from Streptomyces sp.

buffered aqueous glycerol solution, ≥15 units/mg protein (E1%)

Synonym: Chitosan N-acetylglucosaminohydrolase

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Properties

Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Carbohydrate Hydrolysis,
form   buffered aqueous glycerol solution
concentration   ≥0.5 mg/mL
solubility   0.1 M acetate buffer, pH 5.5: soluble
storage temp.   −20°C

Description

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Preparation Note

Soluble in 10mL of 0.1 M Acetate buffer at pH 5.5

Unit Definition

One unit will liberate 1.0 μmole of reducing sugar (measured as D-glucosamine equivalents) from chitosan per minute at pH 5.5 at 37 °C.

Physical form

Solution in 50% glycerol containing 50 mM sodium acetate

Application

Chitosanase from Sigma has been used for polarized light-stimulated, enzymatic hydrolysis of chitin and chitosan.

Biochem/physiol Actions

Chitosanase catalyzes the endohydrolysis of β-1,4-linkages between D-glucosamine (GlcN-GlcN) residues in chitosan. The enzyme from Streptomyces has been reported to also hydrolyze the GlcNAc-GlcN linkage in partially acetylated chitosan.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

Protocols

Enzymatic Assay of Chitosanase from Streptomyces Species

This procedure applies to Sigma-Aldrich Product Number, C0794. This procedure is NOT to be used to assay Chitosanase from Streptomyces griseus, Sigma-Aldrich Product Number, C9830.
Keywords: Biochemistry, Enzyme activity, Microbiology

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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