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C0853 Sigma

Anti-Crk-II antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, CR-CR,
species reactivity   chicken, human, mouse
application(s)   microarray: suitable
  western blot: 1:3,000 using whole extract of the Burkitt lymphoma Raji cell line
  western blot: 1:5,000 using whole extract of chicken fibroblasts
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 38 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CRK(1398)
mouse ... Crk(12928)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide K-THVRLLDQQNPDEDFS corresponding to the C-terminal of human Crk-II (amino acids 289-304 with N-terminally added lysine), conjugated to KLH. The sequence is identical in human, mouse and chicken Crk-II and highly conserved (single amino acid substitution) in rat Crk-II. This sequence is absent in Crk-I isoform and has limited homology (60%) to Crk-L..

General description

Crk proteins belong to a family of adaptor proteins with distinct Src homology (SH) domains, SH2 and SH3. Crk-I, Crk-II and CrkL are the cellular homologs of oncogene v-Crk of avian sarcoma virus. While Crk-I and Crk-II are the products of alternate gene splicing, Crk-L is encoded by a different gene although it shares 60% homology with Crk-II. The Crk proteins are involved in many signal transduction pathways. The SH2 domain of Crk proteins interact with several tyrosine kinases such as EGFR, Cbl, p130cas, paxillin and IRS-1. In humans, both SH2 and SH3 domains of Crk proteins are essential for neuronal differentiation of PC-12 cells, play active role in NGF and p21ras signalling.
Anti-Crk-II specifically recognizes human and chicken Crk-II (38 kDa).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-Crk-II antibody may be used at a working dilution of 1:5000 in whole extract of chicken fibroblasts. It may be used for detection of Crk-II in whole extract of Raji cell line (Burkitt lymphoma). It is also suitable for protein microarray.

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