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C0858 Sigma

Cyclooxygenase 2 human

≥70% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, aqueous solution, ≥8000 units/mg protein

Synonym: COX-2, Prostaglandin H synthase 2

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Properties

Related Categories Application Index, Arachidonic Acid Cascade, Arachidonic Acid Cascade Enzymes, Biochemicals and Reagents, Cell Biology,
recombinant   expressed in baculovirus infected Sf21 cells
assay   ≥70% (SDS-PAGE)
form   aqueous solution
mol wt   dimer subunit mol wt 70-74 kDa
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... PTGS2(5743)

Description

Biochem/physiol Actions

COX-2 catalyzes the conversion of arachidonic acid to prostaglandin H2 (the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins). It is the inducible form of cyclooxygenase, induced by phorbol esters, lipopolysaccharides, and cytokines. COX-2 is upregulated in the neovasculature of malignant tumors. Inhibition of COX-2 with nonsteroidal anti-inflammatory drugs (NSAIDs) is associated with decreased tumor growth.

Other Notes

Tagged with a six-residue histidine sequence near the N-terminus.

Substrates

KM for arachidonate = 6.5 μM. Exhibits activity and sensitivity to NSAIDs similar to the non-tagged enzyme.

Unit Definition

One unit will consume 1.0 nanomole of oxygen per minute at pH 8 at 37 °C.

Physical form

Solution in 80 mM Tris-HCl, pH 8.0 with 0.1% TWEEN® 20 and 300 μM diethyldithiocarbamate and 10% glycerol.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

Articles

Omega-3 Fatty Acids and Heart Disease

The potential for the prevention and treatment of cardiovascular disease through increased dietary intake of omega-3 (w-3) fish oils is not a recent scientific discovery. A historically important stu...
BioFiles 2007, 2.2, 14.
Keywords: Anti-inflammatory agents, Biochemistry, Cardiovascular, Clinical, Degradations, Esterifications, Immunology, Inflammation, Lipid Metabolism, Metabolism, Metabolites, Methods, Nutrition, Oxidations, Transcription

Peer-Reviewed Papers
15

References

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