|Related Categories||Application Index, Arachidonic Acid Cascade, Biochemicals and Reagents, Cell Biology, Cell Signaling Enzymes,|
|recombinant||expressed in baculovirus infected Sf21 cells|
|mol wt||dimer subunit mol wt 70-74 kDa|
|shipped in||dry ice|
|Gene Information||human ... PTGS2(5743)|
COX-2 catalyzes the conversion of arachidonic acid to prostaglandin H2 (the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins). It is the inducible form of cyclooxygenase, induced by phorbol esters, lipopolysaccharides, and cytokines. COX-2 is upregulated in the neovasculature of malignant tumors. Inhibition of COX-2 with nonsteroidal anti-inflammatory drugs (NSAIDs) is associated with decreased tumor growth.
Tagged with a six-residue histidine sequence near the N-terminus.
KM for arachidonate = 6.5 μM. Exhibits activity and sensitivity to NSAIDs similar to the non-tagged enzyme.
One unit consumes one nanomole of oxygen per minute at 37 °C in 0.1 M Tris-HCl buffer, pH 8, containing 100 μM arachidonate, 5 mM EDTA, 2 mM phenol, and 1 μM hematin.
Solution in 80 mM Tris-HCl, pH 8, with 0.1% TWEEN® 20 and 300 μM diethyldithiocarbamate.
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Keywords: Anti-inflammatory agents, Biochemistry, Cardiovascular, Clinical, Degradations, Esterifications, Immunology, Inflammation, Lipid Metabolism, Metabolism, Metabolites, Nutrition, Oxidations, Transcription
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