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C0875 Sigma

Calcein

Used for the fluorometric determination of calcium and EDTA titration of calcium in the presence of magnesium.

Synonym: Bis[N,N−bis(carboxymethyl)aminomethyl]fluorescein, Fluorescein-bis(methyliminodiacetic acid), Fluorexon

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

5, 10, 25 g in glass bottle

Biochem/physiol Actions

Calcein is a calcium-dependent fluorescent molecule. It is mainly required to study bone metabolism under in vivo conditions and helps in the staining of pit area under in vitro conditions. Calcein is used for the fluorometric determination of calcium and EDTA (ethylenediaminetetraacetic acid) titration of calcium.

Price and Availability


Tissue Diagnostics

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
What are the excitation and emission wavelengths of Calcein, Product C0875?
Conn's Biological Stains, 10th Edition states excitation is at 494 nm in aqueous solutions at pH 9; the emission maximum is 517 nm. Depending upon the pH of the solution, slightly different values have been reported in the literature.
Is there a reference for using Calcein, Product C0875, to study bone growth?
Clement-Lacroix, P., et al. Osteoblasts are a new target for prolactin: analysis of bone formation in prolactin receptor knockout mice. Endocrinology, 140, 96-105 (1999).
What is the extinction coefficient of Calcein, Product C0875?
For samples in 1 M sodium hydroxide: E1% = 1040 at 500-504 nm; E1% = 466 at 242-245 nm.
What is the solubility of Calcein, Product C0875?
Calcein is soluble at a concentration of 50 mg/mL in 1M sodium hydroxide, yielding clear orange to brown solution.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

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Peer-Reviewed Papers
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References

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