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C0978 Sigma

Anti-Crk-L antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, CR-CR,
species reactivity   mouse, human
application(s)   immunoprecipitation: 5 μg using 20 μg of a human A431 cell lysate
  microarray: suitable
  western blot: 1:1,000-1:5,000 using using human A431 and human Raji cell lysate, respectively
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 38 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CRKL(1399)
mouse ... Crkl(12929)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

A synthetic peptide corresponding to amino acids 283-303 of human Crk-L, conjugated to KLH. The sequence is highly conserved in mouse Crk-L (single amino acid substitution).

General description

CrkL is a 39kD adaptor protein plays a crucial role in the early steps of IL-2 signal transduction. It also facilitates signaling of β-integrin, B-cell or T-cell receptors. Anti-Crk-L antibody can be used in immunoprecipitation for detecting and localizing CrkL. Rabbit anti-Crk-L antibody reacts specifically with CrkL (38 kD) of human.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Application

Anti-Crk-L antibody can be used in western blotting (diluted 1: 1000, 1:5000) using human epidermal carcinoma A431 cell line and Burkitt lymphoma Raji cell line.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

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