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C0979 Sigma

Monoclonal Anti-Catalase antibody produced in mouse

~2 mg/mL, clone CAT-505, purified immunoglobulin, buffered aqueous solution



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies to Cell and Organelle Proteins,
species reactivity   human, mouse, bovine, rat
application(s)   immunocytochemistry: suitable
  immunohistochemistry: suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 0.5-1 μg/mL using a whole extract of HepG2 cells
clone   CAT-505, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 60 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CAT(847)
mouse ... Cat(12359)
rat ... Cat(24248)
biological source   mouse
conjugate   unconjugated



human erythrocyte catalase

General description

Catalase is one of the most efficient and common enzyme found in nearly all living organisms exposed to oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). In most mammalian cell types catalase is localized in peroxisomes which is a tertrameric haemin-enzyme consisting of 4 identical tetrahedrally arranged subunits of 60kDa. It contains four ferriprotoporphyrin groups per molecule having a molecular mass of 240kDa.

Physical form

Solution in 0.01 M phosphate buffered saline, pH7.4, containing 15 mM sodium azide

Preparation Note

Purified from culture supernatant of hybridoma cells grown in a bioreactor.


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (5 papers)

Monoclonal Anti-Catalase antibody produced in mouse is suitable for the following:
• Immunocytochemistry
• Immunohistochemistry
• Indirect ELISA
• Microarray
• Western blotting at a concentration of 0.5-1μg/mL using whole extract of HepG2 cells

Biochem/physiol Actions

Catalase reacts with hydrogen peroxide, a reactive oxygen species, activating its decomposition into water and molecular oxygen and it reacts with hydrogen donors. It functions as a natural anti-oxidant, protecting cells against oxidative damage of proteins, lipids and nucleic acids. Catalase also plays a role in gene expression and apoptosis. Antioxidants have been identified in association with a variety of diverse cellular functions including growth control, proliferation, differentiation, immune response, tumor promotion, apoptosis and activation of viruses.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles


esiRNA Knockdown Efficiency Tested by Western Blotting

A valuable measure of the knock-down potency of any RNAi experiment is the reduction in protein level. Displayed below are the knock-down rates of  selected esiRNAs using quantitative western blot an...
Keywords: Cell proliferation, Gene expression, PAGE, Reductions, Transfection, Western blot

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Peer-Reviewed Papers


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