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C0998 Sigma

Anti-CLC (76-90) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-Eosinophil lysophospholipase, Anti-LGALS10, Anti-LPPL_HUMAN

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Properties

Related Categories Alphabetical Index, Antibodies, CI-CN, Primary Antibodies
species reactivity   human
application(s)   western blot: 1:500-1:2,000
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt ~16 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CLC(1178)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 76-90 of human CLC

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-CLC (76-90) antibody produced in rabbit is suitable for western blotting at a dilution of 1:500-1:2,000.
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about the Sigma Life Science and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Biochem/physiol Actions

Eosinophil lysophospholipase (Charcot-Leyden crystal protein/galactin 10) is an enzyme encoded by the CLC gene in humans. The protein is a lysophospholipase enzyme expressed in eosinophils and basophils. It acts on biological membranes to regulate the multifunctional lysophospholipids and also has a role in role in the biology of inflammation.

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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