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C100 Sigma

Catalase from bovine liver

aqueous suspension, 40,000-60,000 units/mg protein (E1%/405)

Synonym: H2O2:H2O2 oxidoreductase

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Properties

Related Categories 1.11.1.6 Catalase, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Cell Biology, Cell Signaling and Neuroscience,
form   aqueous suspension
mol wt   tetramer mol wt ~250 kDa
isoelectric point   5.4
shipped in   wet ice
storage temp.   2-8°C
Gene Information   cow ... CAT(280743)

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Components

Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.

Application

Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene expression and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalses have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli.

Biochem/physiol Actions

Catalase catalyzes the degradation of hydrogen peroxide into water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H2O2 molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor.

Caution

Solutions of catalse should not be frozen. Frozen solution will result in a 50-70% loss of activity.

Packaging

1 g in serum bottle

Preparation Note

This product is offered as a catalase suspension in water containing 0.1% (w/v) thymol with activity of 40,000-60,000 units/mg protein. To remove the thymol preservative, the catalase crystals may be pelleted by centrifugation, the supernatant, discarded, the pellet resuspended in water, and then pelleted again. The enzyme is soluble in 50 mM potassium phosphate buffer at 1 mg/mL and pH 7.0.

Unit Definition

One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
Can Product C100, Catalase from bovine liver, be shipped on wet ice or dry ice?
Product No. C100 normally ships at ambient temperature. If desired, it can be shipped on wet ice upon special request. C100 cannot be shipped on dry ice. That is, the suspension can be harmed by freezing.
I see that Product C100, Catalase from bovine liver, has 40,000 to 60,000 units per mg protein. What is a unit, and how was this activity determined?
Here is the link to the enzyme assay procedure, which includes the unit definition: http://www.sigmaaldrich.com/img/assets/18160/Catalase.pdf
Is Product C100, Catalase from bovine liver, a solution or a suspension?
Most of the protein (catalase) in Product No. C100 is a solid in suspension, with a small percentage actually in solution.
Is Product C100, Catalase from bovine liver, sterile?
C100 is not necessarily sterile. But it does contain a preservative (0.1% thymol), which should prevent microbial growth.
What is the catalase concentration of Product C100, Catalase from bovine liver?
There is no “official” specification, but the lot history shows that it has varied from 15-100 mg/mL. The concentration is provided on the certificate of analysis for each lot. Note that most of the catalase is typically a solid in suspension, with a small percentage actually in solution.
Your website shows that Product No. C100 is catalase. But I have some notes that indicate that C100 is your product number for calmidazolium chloride. Which one is right?
C-100 was used as a product number for calmidazolium chloride until about 1999 by Research Biochemicals International (RBI), which is now part of Sigma-Aldrich. Calmidazolium chloride is currently listed under Sigma Product No. C3930. So now we use the C100 only for catalase.
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

Articles

Cellular Derived Blood Proteins and Enzymes

Cell damage is induced by reactive oxygen species (ROS). ROS are either free radicals, reactive anions containing oxygen atoms, or molecules containing oxygen atoms that can either produce free radic...
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Protocols

Enzymatic Assay of Catalase (EC 1.11.1.6)

The continuous spectrophotometric rate reduction determination (A240, Light path = 1 cm) is based on the following reaction:
Keywords: Reductions

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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