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C1059 Sigma

Cloning cylinders, glass

volume 150 μL

Synonym: cloning cylinder, cloning equipment

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Properties

Related Categories Cell Culture, Cell Culture Supplies, Cloning, Core Bioreagents, Equipment,
material   light brown (tinted)
sterility   sterile; electron beam irradiated
packaging   dish of 15 cylinders
mfr. no.   Millipore, TR1004
size   8 mm × 8 mm
volume   150 μL

Description

General description

Individual colonies of transfected cells can be isolated and picked from a plate containing many clones. Isolated clones can be dissociated and passaged free from surrounding cells or pulsed with 50-100 uL growth medium which can be analyzed for secreted products. The sterile cloning cylinders are greased on one end to allow the cylinder to seal to the plate. Cells are then dissociated within the cylinder, resuspeneded, and transferred to a new vessel as a pure colony.
These cloning cylinders are sterlized using electron-beam irradiation. The glass cylinders will display a brown tint after being sterlized at the appropriate dosage level.

Packaging

Packaged in 60 mm dish with silicone grease on one end

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Safety Information

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Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

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