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C1184 Sigma

Cellulase from Aspergillus niger

powder, ≥0.3 units/mg solid

Synonym: 1,4-(1,3:1,4)-β-D-Glucan 4-glucanohydrolase

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Description

Frequently Asked Questions

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Biochem/physiol Actions

Cellulase from Aspergillus niger catalyzes the hydrolysis of endo-1,4-β-D-glycosidic linkages in cellulose, lichenin, barley glucan, and the cellooligosaccharides cellotriose to cellohexaose. It does not cleave cellobiose or p-nitrophenyl-β-D-glucoside. This enzyme will also cleave intact glycosaminoglycan from a core peptide by hydrolyzing the xylosyl serine linkage.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Unit Definition

One unit will liberate 1.0 μmole of glucose from cellulose in one hr at pH 5.0 at 37 °C (2 hr incubation time).

Application

Cellulase from Sigma has been used to study the ability of several of its possible substrates, cellulose, Avicel PH-101, and filter paper, to protect enzyme activity during monogastric diegstion in animal and avian digestive tracts.1

The enzyme has also been approved as a secondary direct food additive as an aid in clam and shrimp processing.

General description

The FDA recognizes cellulase from A. niger as GRAS (Generally Regarded As Safe) if non-pathogenic and non-toxigenic strains, as well as current good manufacturing practices, are used in production.

Price and Availability

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Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



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Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
Hazard Codes (Europe) 
Xn
Risk Statements (Europe) 
42
Safety Statements (Europe) 
22-24-36/37
WGK Germany 
1

Protocols & Articles

Protocols

Enzymatic Assay of Cellulase

3.12 Unit definition: One unit will liberate 1.0 μmole of glucose from cellulose in one hour at pH 5.0 at 37ºC (2 hour incubation time).
Keywords: Extinction coefficient

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Peer-Reviewed Papers

References

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1. Usage of Enzyme Substrate to Protect the Activities of Cellulase, Protease and α-Amylase in Simulations of Monogastric Animal and Avian Sequential Total Tract Digestion Wang HT and Hsu JT Asian. Australas. J. Anim. Sci. 19(8), 1164-1173, (2006)

A single-molecule analysis reveals morphological targets for cellulase synergy. Fox JM, Jess P, Jambusaria RB, et al. Nat. Chem. Biol. 9(6), 356-61, (2013)

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Recyclable thermoresponsive polymer-cellulase bioconjugates for biomass depolymerization. Mackenzie KJ and Francis MB J. Am. Chem. Soc. 135(1), 293-300, (2013)

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Structural, biochemical, and computational characterization of the glycoside hydrolase family 7 cellobiohydrolase of the tree-killing fungus Heterobasidion irregulare. Momeni MH, Payne CM, Hansson H, et al. J. Biol. Chem. 288(8), 5861-72, (2013)

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Formation of highly twisted ribbons in a carboxymethylcellulase gene-disrupted strain of a cellulose-producing bacterium. Nakai T, Sugano Y, Shoda M, et al. J. Bacteriol. 195(5), 958-64, (2013)

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On the safety of Aspergillus niger--a review. Schuster E, Dunn-Coleman N, Frisvad JC, et al. Appl. Microbiol. Biotechnol. 59(4-5), 426-35, (2002)

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G. Okada Meth. Enzymol. 160, 259, (1988)

Substrate specificity and mode of action of a cellulase from Aspergillus niger. Hurst, P.L., et al. Biochem. J. 169, 389-395, (1978)

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Comment on "Revealing nature's cellulase diversity: the digestion mechanism of Caldicellulosiruptor bescii CelA". Gusakov AV Science 344(6184), 578, (2014)

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[Study on preparation of sagittatoside B with epimedin B converted from cellulase]. Xu FJ, Sun E, Zhang ZH, et al. Zhongguo Zhong Yao Za Zhi 39(2), 235-9, (2014)

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[Binding of transcription regulator Clpxoo to promoter of endoglucanase gene engAxoo was inhibited by c-di-GMP in Xanthomonas oryzae pv. oryzae]. Li B, Tian F, Chen H, et al. Wei Sheng Wu Xue Bao 53(11), 1166-71, (2013)

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Adaptive expression of host cell wall degrading enzymes in fungal disease: an example from Fusarium root rot of medicinal Coleus. Bhattacharya A Pak. J. Biol. Sci. 16(24), 2036-40, (2013)

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Microbiology. No barriers to cellulose breakdown. Berlin A Science 342(6165), 1454-6, (2013)

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Screening and identification of newly isolated cellulose-degrading bacteria from the gut of xylophagous termite Microcerotermes diversus (Silvestri). Pourramezan Z, Ghezelbash GR, Romani B, et al. Mikrobiologiia 81(6), 796-802, (2012)

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Cellulases: Cooperative biomass breakdown. Igarashi K Nat. Chem. Biol. 9(6), 350-1, (2013)

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Simplifying cellulase production by using environmental selection pressures and recycling substrate. Lever M, Hoa G, and Cord-Ruwisch R Environ. Technol. 34(1-4), 471-5, (2013)

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[Screening and identifying cellulose degrading bacteria associated with Bursaphelenchus xylophilus and cloning corresponding genes]. Niu Q, Zhang L, Chu X, et al. Wei Sheng Wu Xue Bao 52(11), 1408-14, (2012)

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Combination of liquid hot water pretreatment and wet disk milling to improve the efficiency of the enzymatic hydrolysis of eucalyptus. Weiqi W, Shubin W, and Liguo L Bioresour. Technol. 128, 725-30, (2013)

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[Study on the extraction process of total flavonoids from Psoralea corylifolia by enzyme-assisted technique]. Yan YS, Liao HW, and Guo LB Zhong Yao Cai 35(7), 1144-7, (2012)

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[Isolation, identification and cellulase production of a cellulolytic bacterium from intestines of giant panda]. Fan C, Li S, Li C, et al. Wei Sheng Wu Xue Bao 52(9), 1113-21, (2012)

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[Screening of two straw-cellulose degrading actinomycetes]. Gu W, Zhang F, Xu P, et al. Wei Sheng Wu Xue Bao 52(9), 1085-93, (2012)

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Determination of the structure of an endoglucanase from Aspergillus niger and its mode of inhibition by palladium chloride. Khademi, S., et al. Acta Crystallogr. D Biol. Crystallogr. 58, 660-667, (2002)

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Cleavage of the xylosyl serine linkage between a core peptide and a glycosaminoglycan chain by cellulases. Takagaki, K., et al. J. Biol. Chem. 277, 18397-18403, (2002)

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Small angle X-ray scattering analysis of Clostridium thermocellum cellulosome N-terminal complexes reveals a highly dynamic structure. Currie MA, Cameron K, Dias FM, et al. J. Biol. Chem. 288(11), 7978-85, (2013)

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Phase-diagram-guided method for growth of a large crystal of glycoside hydrolase family 45 inverting cellulase suitable for neutron structural analysis. Nakamura A, Ishida T, Fushinobu S, et al. J. Synchrotron Radiat. 20(Pt 6), 859-63, (2013)

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Endo-(1,4)-β-glucanase gene families in the grasses: temporal and spatial co-transcription of orthologous genes. Buchanan M, Burton RA, Dhugga KS, et al. BMC Plant Biol. 12, 235, (2012)

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Improved lignocellulose conversion to biofuels with thermophilic bacteria and thermostable enzymes. Bhalla A, Bansal N, Kumar S, et al. Bioresour. Technol. 128, 751-9, (2013)

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Enzymatic hydrolysis of microcrystalline cellulose and pretreated wheat straw: a detailed comparison using convenient kinetic analysis. Monschein M, Reisinger C, and Nidetzky B Bioresour. Technol. 128, 679-87, (2013)

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Hybrid cellulase aggregate with a silica core for hydrolysis of cellulose and biomass. Sutarlie L and Yang KL J. Colloid. Interface Sci. 411, 76-81, (2013)

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Regulation of cellulolytic genes by McmA, the SRF-MADS box protein in Aspergillus nidulans. Yamakawa Y, Endo Y, Li N, et al. Biochem. Biophys. Res. Commun. 431(4), 777-82, (2013)

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Cloning and recombinant expression of a cellulase from the cellulolytic strain Streptomyces sp. G12 isolated from compost. Amore A, Pepe O, Ventorino V, et al. Microb. Cell Fact. 11, 164, (2012)

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A cytoplasm-specific activity encoded by the Trithorax-like ATX1 gene. Ndamukong I, Lapko H, Cerny RL, et al. Nucleic Acids Res. 39(11), 4709-18, (2011)

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Revealing nature's cellulase diversity: the digestion mechanism of Caldicellulosiruptor bescii CelA. Brunecky R, Alahuhta M, Xu Q, et al. Science 342(6165), 1513-6, (2013)

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Monitoring real-time enzymatic hydrolysis of Distillers Dried Grains with Solubles (DDGS) by dielectric spectroscopy following hydrothermal pre-treatment by steam explosion. Bryant DN, Firth E, Kaderbhai N, et al. Bioresour. Technol. 128, 765-8, (2013)

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Evidence for transceptor function of cellodextrin transporters in Neurospora crassa. Znameroski EA, Li X, Tsai JC, et al. J. Biol. Chem. 289(5), 2610-9, (2014)

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Global identification of multiple OsGH9 family members and their involvement in cellulose crystallinity modification in rice. Xie G, Yang B, Xu Z, et al. PLoS ONE 8(1), e50171, (2013)

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Purification and characterization of a new β-glucosidase from Penicillium piceum and its application in enzymatic degradation of delignified corn stover. Gao L, Gao F, Zhang D, et al. Bioresour. Technol. 147, 658-61, (2013)

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The effect of low-frequency ultrasound on the activity and efficiency of a commercial cellulase enzyme. Szabó OE and Csiszár E Carbohydr. Polym. 98(2), 1483-9, (2013)

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Crystal structure of endo-1,4-β-glucanase from Eisenia fetida. Arimori T, Ito A, Nakazawa M, et al. J. Synchrotron Radiat. 20(Pt 6), 884-9, (2013)

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Two major facilitator superfamily sugar transporters from Trichoderma reesei and their roles in induction of cellulase biosynthesis. Zhang W, Kou Y, Xu J, et al. J. Biol. Chem. 288(46), 32861-72, (2013)

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Cellulase-lignin interactions-the role of carbohydrate-binding module and pH in non-productive binding. Rahikainen JL, Evans JD, Mikander S, et al. Enzyme Microb. Technol. 53(5), 315-21, (2013)

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Effects of a mono-component endo-xylanase supplementation on the nutritive value of wheat-based broiler diets. Francesch M, Pérez-Vendrell AM, and Broz J Br. Poult. Sci. 53(6), 809-16, (2012)

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Enzymatic properties of Thermoanaerobacterium thermosaccharolyticum β-glucosidase fused to Clostridium cellulovorans cellulose binding domain and its application in hydrolysis of microcrystalline cellulose. Zhao L, Pang Q, Xie J, et al. BMC Biotechnol. 13, 101, (2013)

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The capability of endophytic fungi for production of hemicellulases and related enzymes. Robl D, Delabona Pda S, Mergel CM, et al. BMC Biotechnol. 13, 94, (2013)

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Halostable cellulase with organic solvent tolerance from Haloarcula sp. LLSG7 and its application in bioethanol fermentation using agricultural wastes. Li X and Yu HY J. Ind. Microbiol. Biotechnol. 40(12), 1357-65, (2013)

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Overproduction, purification, crystallization and preliminary X-ray characterization of the C-terminal family 65 carbohydrate-binding module (CBM65B) of endoglucanase Cel5A from Eubacterium cellulosolvens. Venditto I, Baslé A, Luís AS, et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 69(Pt 2), 191-4, (2013)

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Low cost single-step purification of endoglucanase from Aspergillus fumigatus ABK-9. Das A, Paul T, Halder SK, et al. Indian J. Exp. Biol. 51(11), 954-9, (2013)

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Mathematical model-based optimization of physico-enzymatic hydrolysis of Pinus roxburghii needles for the production of reducing sugars. Vats S, Maurya DP, Jain A, et al. Indian J. Exp. Biol. 51(11), 944-53, (2013)

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Hydrolytic Activity and Substrate Specificity of an Endoglucanase from Zea mays Seedling Cell Walls. Hatfield RD and Nevins DJ Plant Physiol. 83(1), 203-7, (1987)

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Study on regulation of growth and biosynthesis of cellulolytic enzymes from newly isolated Aspergillus fumigatus ABK9. Das A, Paul T, Halder SK, et al. Pol. J. Microbiol. 62(1), 31-43, (2013)

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Crystallization and preliminary diffraction analysis of the catalytic domain of major extracellular endoglucanase from Xanthomonas campestris pv. campestris. Rosseto FR, Puhl AC, Andrade MO, et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 69(Pt 2), 137-40, (2013)

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Molecular imprinting and immobilization of cellulase onto magnetic Fe3O4@SiO2 nanoparticles. Li Y, Wang XY, Zhang RZ, et al. J. Nanosci. Nanotechnol. 14(4), 2931-6, (2014)

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Characterization of all family-9 glycoside hydrolases synthesized by the cellulosome-producing bacterium Clostridium cellulolyticum. Ravachol J, Borne R, Tardif C, et al. J. Biol. Chem. 289(11), 7335-48, (2014)

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Purification and characterization of a carboxymethyl cellulase from Artemia salina. Zin HW, Park KH, and Choi TJ Biochem. Biophys. Res. Commun. 443(1), 194-9, (2014)

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Isolation, identification, and media optimization of high-level cellulase production by Bacillus sp. BCCS A3, in a fermentation system using response surface methodology. Kazemi A, Rasoul-Amini S, Shahbazi M, et al. Prep Biochem Biotechnol. 44(2), 107-18, (2014)

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Cell dispersion culture for the effective growth of Humicola insolens and efficient enzyme production. Matsumoto H, Koganei K, Nishida N, et al. J. Biosci. Bioeng. 117(3), 257-62, (2014)

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Functional characterization of two M42 aminopeptidases erroneously annotated as cellulases. Dutoit R, Brandt N, Legrain C, et al. PLoS ONE 7(11), e50639, (2012)

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Improved procedures for preparation and characterization of Myrothecium cellulase. 3. Molecular weight, amino acid composition, terminal residues, and other properties. DATTA PK, HANSON KR, and WHITAKER DR Can. J. Biochem. Physiol. 41, 697-705, (1963)

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Improved procedures for preparation and characterization of myrothecium cellulase. 2. Purification procedures. WHITAKER DR, HANSON KR, and DATTA PK Can. J. Biochem. Physiol. 41, 671-96, (1963)

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Partial purification and some properties of a cellulase from Helix pomatia. MYERS FL and NORTHCOTE DH Biochem. J. 71(4), 749-56, (1959)

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