|Related Categories||3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Carbohydrate Hydrolysis,|
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Cellulase from Aspergillus niger catalyzes the hydrolysis of endo-1,4-β-
One unit will liberate 1.0 μmole of glucose from cellulose in one hr at pH 5.0 at 37 °C (2 hr incubation time).
Cellulase from Sigma has been used to study the ability of several of its possible substrates, cellulose, Avicel PH-101, and filter paper, to protect enzyme activity during monogastric diegstion in animal and avian digestive tracts.1
The enzyme has also been approved as a secondary direct food additive as an aid in clam and shrimp processing.
The FDA recognizes cellulase from A. niger as GRAS (Generally Regarded As Safe) if non-pathogenic and non-toxigenic strains, as well as current good manufacturing practices, are used in production.
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powder, off-white, ~0.8 U/mg
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Certificate of Analysis
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|Precautionary statements||P261-P342 + P311|
|Personal Protective Equipment||dust mask type N95 (US), Eyeshields, Faceshields, Gloves|
|Hazard Codes (Europe)||Xn|
|Risk Statements (Europe)||42|
|Safety Statements (Europe)||22-24-36/37|
3.12 Unit definition: One unit will liberate 1.0 μmole of glucose from cellulose in one hour at pH 5.0 at 37ºC (2 hour incubation time).
Keywords: Extinction coefficient
From our library of Related Content, Sigma-Aldrich presents Enzyme Explorer: the most comprehensive source of enzymes, substrates, activators, & inhibitors.
Keywords: Cell culture, Cell disruption, Cell signaling, Diagnostic, Digestions, Drug discovery, Functional genomics, Gene expression, Genomics, Metabolic Pathways, Molecular biology, Neuroscience, Proteomics
1. Usage of Enzyme Substrate to Protect the Activities of Cellulase, Protease and α-Amylase in Simulations of Monogastric Animal and Avian Sequential Total Tract Digestion Wang HT and Hsu JT Asian. Australas. J. Anim. Sci. 19(8), 1164-1173, (2006)
Enzymatic hydrolysis of microcrystalline cellulose and pretreated wheat straw: a detailed comparison using convenient kinetic analysis. Monschein M, Reisinger C, and Nidetzky B Bioresour. Technol. 128, 679-87, (2013)
Monitoring real-time enzymatic hydrolysis of Distillers Dried Grains with Solubles (DDGS) by dielectric spectroscopy following hydrothermal pre-treatment by steam explosion. Bryant DN, Firth E, Kaderbhai N, et al. Bioresour. Technol. 128, 765-8, (2013)
Combination of liquid hot water pretreatment and wet disk milling to improve the efficiency of the enzymatic hydrolysis of eucalyptus. Weiqi W, Shubin W, and Liguo L Bioresour. Technol. 128, 725-30, (2013)
Structural, biochemical, and computational characterization of the glycoside hydrolase family 7 cellobiohydrolase of the tree-killing fungus Heterobasidion irregulare. Momeni MH, Payne CM, Hansson H, et al. J. Biol. Chem. 288(8), 5861-72, (2013)
Small angle X-ray scattering analysis of Clostridium thermocellum cellulosome N-terminal complexes reveals a highly dynamic structure. Currie MA, Cameron K, Dias FM, et al. J. Biol. Chem. 288(11), 7978-85, (2013)
Crystallization and preliminary diffraction analysis of the catalytic domain of major extracellular endoglucanase from Xanthomonas campestris pv. campestris. Rosseto FR, Puhl AC, Andrade MO, et al. Acta Crystallogr. F, Struct. Biol. Cryst. Commun. 69(Pt 2), 137-40, (2013)
Overproduction, purification, crystallization and preliminary X-ray characterization of the C-terminal family 65 carbohydrate-binding module (CBM65B) of endoglucanase Cel5A from Eubacterium cellulosolvens. Venditto I, Baslé A, Luís AS, et al. Acta Crystallogr. F, Struct. Biol. Cryst. Commun. 69(Pt 2), 191-4, (2013)
Screening and identification of newly isolated cellulose-degrading bacteria from the gut of xylophagous termite Microcerotermes diversus (Silvestri). Pourramezan Z, Ghezelbash GR, Romani B, et al. Mikrobiologiia 81(6), 796-802, (2012)
Improved procedures for preparation and characterization of Myrothecium cellulase. 3. Molecular weight, amino acid composition, terminal residues, and other properties. DATTA PK, HANSON KR, and WHITAKER DR Can. J. Biochem. Physiol. 41, 697-705, (1963)
G. Okada Meth. Enzymol. 160, 259, (1988)
Determination of the structure of an endoglucanase from Aspergillus niger and its mode of inhibition by palladium chloride. Khademi, S., et al. Acta Crystallogr. D, Biol. Crystallogr. 58, 660-667, (2002)
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