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C1261 Sigma

Carboxypeptidase A−Agarose

ammonium sulfate suspension, ≥6 units/mL packed gel, 25 °C, carboxypeptidase A from bovine pancreas

Synonym: Carboxypolypeptidase, Peptidyl-L-amino-acid hydrolase

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Description

Unit Definition

One unit will hydrolyze 1.0 μmole of hippuryl-L-phenylalanine per min at pH 7.5 at 25 °C.

Physical form

Suspension in 2.0 M (NH4)2SO4, pH 7

Biochem/physiol Actions

Carboxypeptidase as isolated from bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by β-phenylpropionate and indole acetate.

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Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Enzymatic Assay of Carboxypeptidase A

To standardize a procedure for the assay of Carboxypeptidase A at Sigma-Aldrich, St. Louis.
Keywords: Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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related product

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CS1130 Carboxypeptidase A Assay Kit, sufficient for 200 assays (96-well plates), sufficient for 40 assays (1 mL cuvettes)

substrate

Product #

Description

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H6875 Hippuryl-L-phenylalanine

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