Synonym: H2O2:H2O2 oxidoreductase
|Related Categories||220.127.116.11 Catalase, 1.x.x.x Oxidoreductases, Application Index, Biochemicals and Reagents, Cell Culture,|
|mol wt||tetramer mol wt ~250 kDa|
|suitability||suitable for cell culture|
|Gene Information||cow ... CAT(280743)|
Protein determined by biuret.
Catalase is used to eliminate H2O2 from enzymatic oxidation reactions.1,2
Activates the decomposition of hydrogen peroxide, Natural antioxidant used to study roles of reactive oxygen species in gene expression and apoptosis.
Catalase catalyzes the decomposition of hydrogen peroxide into water and oxygen.
Catalase from bovine liver catalyzes the decomposition of H2O2 into water and oxygen.1,2
One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 conc. falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.
Catalase from bovine liver is a tetramer consisting of four equal subunits with a molecular weight of 60 kDa each. Each subunit contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group.
Customers Also Viewed
ammonium sulfate suspension, ≥4,000 units/mg protein
lyophilized powder, 2,000-5,000 units/mg protein
aqueous suspension, 10,000-40,000 units/mg protein
lyophilized powder, ≥10,000 units/mg protein
aqueous solution, ≥30,000 units/mg protein
Certificate of Analysis
Certificate of Origin
The continuous spectrophotometric rate reduction determination (A240, Light path = 1 cm) is based on the following reaction:
From our library of Related Content, Sigma-Aldrich presents Enzyme Explorer: the most comprehensive source of enzymes, substrates, activators, & inhibitors.
Keywords: Cell culture, Cell disruption, Cell signaling, Diagnostic, Digestions, Drug discovery, Functional genomics, Gene expression, Genomics, Metabolic Pathways, Molecular biology, Neuroscience, Proteomics
Therapeutic effect of magnesium sulphate on carbon monoxide toxicity-mediated brain lipid peroxidation. Yavuz Y, Mollaoglu H, Yürümez Y, et al. Eur. Rev. Med. Pharmacol. Sci. 17 Suppl 1, 28-33, (2013)
Biomonitoring study of an estuarine coastal ecosystem, the Sacca di Goro lagoon, using Ruditapes philippinarum (Mollusca: Bivalvia). Sacchi A, Mouneyrac C, Bolognesi C, et al. Environ. Pollut. 177, 82-9, (2013)
[The blood biochemical indicators as possible markers of destruction of kerato-hyaline tissue of nails in patients with onycholysis]. Urazovskaia EV and Mikashinovich ZI Klin. Lab. Diagn. (12), 8-10, (2012)
Spectroscopic determination of the toxicity, absorption, reduction, and translocation of Cr(VI) in two Magnoliopsida species. Montes MO, Peralta-Videa JR, Parsons JG, et al. Int. J. Phytoremediation 15(2), 168-87, (2013)
Epithelial-mesenchymal transition during oncogenic transformation induced by hexavalent chromium involves reactive oxygen species-dependent mechanism in lung epithelial cells. Ding SZ, Yang YX, Li XL, et al. Toxicol. Appl. Pharmacol. 269(1), 61-71, (2013)
Interactions between A(2A) adenosine receptors, hydrogen peroxide, and KATP channels in coronary reactive hyperemia. Sharifi-Sanjani M, Zhou X, Asano S, et al. Am. J. Physiol. Heart Circ. Physiol. 304(10), H1294-301, (2013)
Melatonin protects rat thymus against oxidative stress caused by exposure to microwaves and modulates proliferation/apoptosis of thymocytes. Sokolovic D, Djordjevic B, Kocic G, et al. Gen. Physiol. Biophys. 32(1), 79-90, (2013)
Study of the physiological characteristics of the medicinal mushroom Trametes pubescens (higher Basidiomycetes) during the laccase-producing process. Si J and Cui BK Int. J. Med. Mushrooms 15(2), 199-210, (2013)
[Influence of ethanol-metabolising systems on intensity of lipid peroxidation processes in gastrointestinal tract of rats]. Petushok NÉ, Grokhovskaia TCh, Mel'nichenko NG, et al. Biomed. Khim. 59(1), 76-80, (2013)
[Development of Scotch pine seedlings and functioning of antioxidant systems under the chronic action of lead ions]. Ivanov IuV, Savochkin IuV, and Kunetsov VV Izv. Akad. Nauk Ser. Biol. (1), 32-42, (2013)
[Effect of population density on enzymatic activity of antioxidative and phenol oxidase systems of imagoes and nymphs of the marble cockroach Nauphoeta cinerea]. Murzagulov GS, Saltykova ES, Gaĭfullina LR, et al. Zh. Evol. Biokhim. Fiziol. 49(1), 24-9, (2013)
Differential antioxidative response of tolerant and sensitive maize (Zea mays L.) genotypes to drought stress at reproductive stage. Chugh V, Kaur N, Grewal MS, et al. Indian J. Biochem. Biophys. 50(2), 150-8, (2013)
[The antioxidant enzyme activity in mouse liver mitochondria after nanosecond pulsed periodic X-ray exposure]. Kniazeva IR, Ivanov VV, Bol'shakov MA, et al. Radiats. Biol. Radioecol. 53(2), 183-90, (2013)
Gastroprotective effect of an ethanolic extract from Neoglaziovia variegata (Arruda) Mez (Bromeliaceae) in rats and mice. Machado FD, Silva FV, Fernandes HB, et al. Z. Naturforsch. C 68(3-4), 97-107, (2013)
O2 and reactive oxygen species detoxification complex, composed of O2-responsive NADH:rubredoxin oxidoreductase-flavoprotein A2-desulfoferrodoxin operon enzymes, rubperoxin, and rubredoxin, in Clostridium acetobutylicum. Kawasaki S Appl. Environ. Microbiol. 75(4), 1021-9, (2009)
Identification of Francisella tularensis live vaccine strain CuZn superoxide dismutase as critical for resistance to extracellularly generated reactive oxygen species. Melillo AA J. Bacteriol. 191(20), 6447-56, (2009)
Role of the endogenous antioxidant system in the protection of Schistosoma mansoni primary sporocysts against exogenous oxidative stress. Marina de Moraes Mourão et al PLoS Negl. Trop. Dis. 3, e550, (2009)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Need larger quantities for your development, manufacturing or research applications?