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C1345 Sigma

Catalase from bovine liver

powder, suitable for cell culture, 2,000-5,000 units/mg protein

DOWNLOAD MSDS (PDF)

Synonym: H2O2:H2O2 oxidoreductase

Properties

Related Categories 1.11.1.6 Catalase, 1.x.x.x Oxidoreductases, Application Index, Biochemicals and Reagents, Cell Culture,
form   powder
mol wt   tetramer mol wt ~250 kDa
isoelectric point   5.4
suitability   suitable for cell culture
storage temp.   −20°C
Gene Information   cow ... CAT(280743)

Description

Analysis Note

Protein determined by biuret.

Application

Catalase is used to eliminate H2O2 from enzymatic oxidation reactions.1,2

Biochem/physiol Actions

Activates the decomposition of hydrogen peroxide, Natural antioxidant used to study roles of reactive oxygen species in gene expression and apoptosis.

Catalase catalyzes the decomposition of hydrogen peroxide into water and oxygen.

Catalase from bovine liver catalyzes the decomposition of H2O2 into water and oxygen.1,2

Unit Definition

One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 conc. falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.

Physical properties

Catalase from bovine liver is a tetramer consisting of four equal subunits with a molecular weight of 60 kDa each. Each subunit contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group.

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