• USA Home
  • C1370 - COT (30-397), active, GST tagged human

EMAIL THIS PAGE TO A FRIEND
C1370 Sigma

COT (30-397), active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym: EST, FLJ10486, MAP3K8, TPL2, c-COT

Purchase

Properties

Related Categories Active Kinases, Application Index, Biochemicals and Reagents, Cell Biology, Cell Signaling Enzymes,
recombinant   expressed in baculovirus infected Sf9 cells
grade   PRECISIO® Kinase
assay   ≥70% (SDS-PAGE)
form   buffered aqueous glycerol solution
activity   860-1164 nmol/min·mg
mol wt   mol wt ~70 kDa
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... MAP3K8(1326)

Description

Biochem/physiol Actions

COT is an oncogene that can activate both the MAP kinase and JNK kinase pathways. COT activates I?B kinases and induces the nuclear production of NF-?B. C-terminal catalytic domain of KSR2 associates with COT and KSR2 can negatively regulates the kinase activity of COT in vitro. Co-transfection of KSR2 with COT in cells lead to reduced COT-mediated ERK activation and COT-induced IL8 production in a dose-dependent manner. COT is one of the MAP kinase kinase kinases that regulates the ERK1/ERK2 pathway in response to IL-1. Blockage of expression of COT results in failure of IL-1 to induce an increase in IL-8 and MIP-1betamRNA levels.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


All labs need water
Safety & Documentation
Protocols & Articles
Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?