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C1562 Sigma

Freezing container, Nalgene® Mr. Frosty

H × diam. 86 mm × 117 mm

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Properties

Related Categories Cryo Containers, Cryoware, Labware, Manufacturer Browser, Nalgene,
mfr. no.   Nalgene
H × diam.   86 mm × 117 mm

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Compatibility

Holds 18 x 1.2 and 2.0 mL cryovials

General description

Polycarbonate container, blue high-density polyethylene closure, white high-density polyethylene vial holder, and foam insert.

Provides the critical, repeatable, 1°C/min cooling rate required for successful cryopreservation of cells. Easy to use in any mechanical freezer. Container is imprinted with graphic instructions. Requires only isopropyl alcohol. Rigid vial holder keeps vials from contacting alcohol, preventing contamination by wicking. Vial holder floats to allow thawing in a water bath. Numbers molded for identification.

All components withstand repeated freeze/thaw cycles. Stackable.

Application

Freezing container, Nalgene® Mr. Frosty may be used for freezing plant cells, protoplasts , PBMC (peripheral blood mononuclear cell) .

Legal Information

Nafion is a registered trademark of The Chemours Company FC, LLC

Nalgene is a registered trademark of Thermo Fisher Scientific or its subsidiaries

Protocols & Applications

Protocol for cyropreservation

Price and Availability

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
What alcohol should I use with Mr. Frosty, Product C1562 (Freezing container, Nalgene®)?
We recommend using 2-propanol, commonly known as isopropanol. Among our product listings for 2-propanol we offer Product No. 190764.
How do I freeze my cells in a Nalgene® Mr. Frosty, freezing container, product C1562?
Listed below are instructions for the Nalgene® Mr. Frosty freezing container. Fill the container with room-temperature 100% isopropyl alcohol to the indicated fill line and replace the foam insert and tube holder.   Insert your sample tubes into the tube holder and place the Mr. Frosty container in -70°C freezer for a minimum of 4 hours. Remove frozen tubes and place in long term storage freezer, at or below -130°C. Note: When not in use, the Mr. Frosty container should be stored at room temperature.
What is the rate of cooling of the cell suspension in Product C1562, Freezing container, Nalgene®?
The rate of cooling of 1°C/minute is based on the use of isopropanol as the alcohol bath.
Can I use Product C1562, Freezing container, Nalgene®, with 5 mL freezing vials?
Product No. C1562 is designed for use with 1.2 and 2 mL freezing vials.  It cannot be used with 5 mL freezing vials.  Nalgene no longer offers the Mr. Frosty in the 5 mLsize.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

Protocols

Cryopreservation of Cell Lines

The protocol below describes the use of passive methods involving an electric -80°C freezer for the cryopreservation of cell cultures. ECACC routinely use a programmable rate controlled freezer. This...
Cook Book Volume 12
Keywords: Cell culture, Cryopreservation, Phase transitions

Protocol for the Cryopreservation of Cell Lines

This protocol is recommended by the European Collection of Authenticated Cell Cultures (ECACC, a part of Public Health England) for the cryopreservation of its cell lines. This protocol employs the u...
Keywords: Cryopreservation, Phase transitions

Peer-Reviewed Papers
15

References

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