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C1748 Sigma

Anti-CLK1 (86-100) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-CDC-like kinase 1, Anti-CLK/STY, Anti-CLK, Anti-STY



Related Categories Alphabetical Index, Antibodies, CI-CN, Primary Antibodies
species reactivity   human
application(s)   western blot: 1:500-1:2,000
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt ~57 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CLK1(1195)
biological source   rabbit
conjugate   unconjugated



synthetic peptide corresponding to amino acids 86-100 of human CLK1

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Anti-CLK1 (86-100) antibody produced in rabbit is suitable for western blotting at a dilution of 1:500-1:2000.
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about the Sigma Life Science and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Biochem/physiol Actions

CDC-like kinase 1 (CLK1) is an enzyme encoded by the CLK1 gene in humans. It belongs to the CDC2-like (or LAMMER) family of dual specificity protein kinases. It is also referred as CLK and STY. Clk/Sty belongs to Clk/Sty family of dual-specificity kinases. It is autophosphorylated using both direct kinase assays and SR protein-dependent splicing assays on both Ser/Thr and Thr residues. Clk/Sty recognize different SR proteins, which is influenced by the extent and nature of autophosphorylation. It is found in the nucleus of several different cell types. CLK expression had distinct effects on HIV-1, CLK1 enhances Gag production while CLK2 inhibits the virus.

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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SAB1300108 Anti-CLK1 (N-term) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

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