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C1801 Sigma

Monoclonal Anti-Cytokeratin, pan antibody produced in mouse

clone PCK-26, ascites fluid

Synonym: Monoclonal Anti-pan-Cytokeratin

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytokeratins,
species reactivity   lizard, goat, bovine, human, hamster, rat, carp, guinea pig, pig, rabbit, sheep, mouse, chicken, snake, feline, canine
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300
  immunohistochemistry (frozen sections): suitable
  indirect immunofluorescence: 1:300 using protease-idgested, formalin-fixed, paraffin-embedded sections of human or animal tissues
  western blot: suitable
clone   PCK-26, monoclonal
antibody form   ascites fluid
isotype   IgG1
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
biological source   mouse
conjugate   unconjugated

Description

Immunogen

cytokeratin from human epidermis.

General description

Cytokeratins, a group comprising at least 29 different proteins are characteristic of epithelial and trichocytic cells. Cytokeratin 1, 5, 6, and 8 are members of the type II neutral-to-basic subfamily. Cytokeratin peptide 1 (68 kDa) is expressed together with cytokeratin 10 in the suprabasal cell layers or the differentiation compartment of the epidermis. Its expression increases with epidermal maturation and it is modified post-translationally in the terminally differentiated keratinocytes of the stratum corneum. Cytokeratin peptide 5 (58 kDa) is the primary type II keratin in stratified epithelia while cytokeratin type 8 (52 kDa) is a major type II keratin in simple epithelia. Cytokeratin 6 (56 kDa) is a "hyperproliferation" cytokeratin expressed in tissues with natural or pathological high turnover.

Specificity

The antibody recognizes an epitope located on the Type II cytokeratins 1, 5, 6, and 8. PCK-26 is a broad spectrum antibody which reacts specifically with a variety of normal, reactive, and neoplastic epithelial tissues. The antibody reacts with simple, cornifying, and non-cornifying squamous epithelia and pseudostratified epithelia.

Application

Monoclonal antibodies to cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti-Cytokeratin, pan is a broadly reactive group-type antibody which recognizes an epitope present in most human epithelial tissues. It facilitates the typing of normal, metaplastic and neoplastic cells and it may aid in the discrimination of carcinomas and non-epithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micro-metastases in lymph nodes and other tissues and for determining the origin of poorly differentiated tumors.
Monoclonal Anti-Cytokeratin, pan may be used for the localization of cytokeratins using various immunochemical assays such as immunoblotting, dot blotting, and immunohistochemstry (immunofluorescence and immunoenzymatic staining).
A minimum antibody titer of 1:300 was determined by indirect immunofluorescent staining of protease digested, formalin-fixed, paraffin-embedded sections of human or animal tissues.

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RIDADR  NONH for all modes of transport
WGK Germany  3
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
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References

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