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C1805 Sigma

Monoclonal Anti-CD4 antibody produced in mouse

clone Q4120, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-CD4

Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Cell Biology, Antibodies for Immune Cell Signaling and Blood,
species reactivity   human
application(s)   flow cytometry: 5 μL using 1 × 106 cells
clone   Q4120, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 59 kDa
shipped in   wet ice
storage temp.   2-8°C
Gene Information   human ... CD4(920)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells.

Antigen Background

CD4 is a single chain transmembraneous glycoprotein from the immunoglobulin superfamily. It is expressed on the helper/inducer T subset, on most medullary thymocytes, on microglial, dendritic and on some malignancies of T cell origin. The antigen binds to MHC class II molecules and is associated with p56lck protein tyrosine kinase.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Specificity

Recognizes the CD4. The epitope recognized by the Q4120 clone is located on 1-183 aa and is sensitive to formalin fixation and paraffin embedding. 5th Workshop: code no. CD04.11

Application

Monoclonal Anti-CD4 antibody produced in mouse is suitable for flow cytometry at a concentration of 5μL using 1 × 106 cells.

Biochem/physiol Actions

CD4 (cluster of differentiation 4) is a glycoprotein found on the surface of immune cells such as T-helper cells, monocytes, macrophages and dendritic cells. CD4 gene is located at chromosome 12p13. Stable CD4 cell expression and local immune activation may promote viral replication, genital shedding and risk of sexual HIV transmission in the lower female genital tract. Patients with infectious and chronic inflammatory diseases are detected with higher level of soluble CD4 (sCD4). It may act as an important parameter for rheumatoid arthritis (RA) disease progression. CD4+ T cells are critical for the pathogenesis of myocarditis. CD4+ CD25+ regulatory T cells (Tregs) may control the development and progression of autoimmunity by suppressing auto-reactive effector T cells.

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