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C1821 Sigma

Monoclonal Anti-Pan Cadherin antibody produced in mouse

clone CH-19, ascites fluid

Synonym: Monoclonal Anti-Pan Cadherin

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Adhesion Molecules and Related Proteins, Antibodies to Cell Membrane,
species reactivity   hamster, mouse, rat, bovine, feline, chicken, human, canine, sheep, frog, guinea pig, pig, snake, Psammomys (sand rat), goat, rabbit
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested animal heart sections
  indirect immunofluorescence: 1:500 using cultured MDBK cells
  microarray: suitable
  western blot: suitable
clone   CH-19, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 135 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CDH1(999), CDH2(1000), CDH3(1001)
mouse ... Cdh1(12550), Cdh2(12558), Cdh3(12560)
rat ... Cdh1(83502), Cdh2(83501), Cdh3(116777)
biological source   mouse
conjugate   unconjugated
Featured Industry   Research Pathology

Description

Immunogen

synthetic peptide corresponding to the C-terminal amino acids of chicken N-Cadherin with an extra N-terminal lysine residue (24 amino acids) coupled to KLH.

General description

Cadherins are members of a multigene family of single chain glycoprotein receptors mediating Ca2+ -dependent cell-cell adhesion. The N-terminal part of these molecule is exposed on the external cell surface and contains the putative homophilic binding sites. This is followed by a typical single transmembrane sequence and usually a cytoplasmic C-terminal tail which mediates interaction with the microfilament system through molecules such as catenins, plakoglobin, vinculin, and α-actinin.
Cadherins that are primarily located in areas of cell-cell contacts are involved in selective cell sorting and in the mechanical cytoplasmic response. They are implicated in morphogenetic processes, intercellular signalling and in tumor invasiveness and metastasis.
Multiple cadherins have been characterized from diverse species and tissues including E-Cadherin, N-Cadherin (A-CAM), P-Cadherin, V-Cadherin, R-Cadherin and T-Cadherin. Specific polyclonal antibodies against a highly conserved sequence from the cytoplasmic C-terminal of N-Cadherin has been prepared.

Specificity

Detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, stains adherens type cell-cell junctions regardless of their cadherin type.

Monoclonal Anti-Pan Cadherin antibody reacts extensively with all known members of the cadherin family. The cadherins migrate in the molecular weight range of 125 to 140 kDa and are tissue dependent. The antibody reacts using frozen sections, protease-digested, formalin-fixed paraffin-embedded, Bouin′s-fixed and acetone-fixed tissue sections. It reacts with cultured cell line preparations. Cross-reaction has been observed with human, rabbit, bovine, goat, sheep, dog, pig, guinea pig, hamster, cat, rat, mouse, psammomys, chicken, frog and snake.

Application

Monoclonal Anti-Pan Cadherin may be used for: immunochemical and immunocytochemical detection of members of the cadherin family in normal and neoplastic cells and tissues; identification of genetically engineered proteins containing the C-terminal cadherin tail; screening of cDNA expression libraries for identification of novel members of the cadherin family and demonstration of adherens-type cell-cell junctions irrespective of their cadherin subtype.

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