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C1926 Sigma

Monoclonal Anti-Collagen, Type IV antibody produced in mouse

clone COL-94, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells, Antibodies for Mesenchymal Stem Cells,
species reactivity   human
application(s)   dot blot: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested sections of human tissue
  immunohistochemistry (frozen sections): suitable
  indirect ELISA: suitable
  microarray: suitable
clone   COL-94, monoclonal
antibody form   ascites fluid
isotype   IgG1
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... COL4A1(1282), COL4A3(1285), COL4A4(1286), COL4A5(1287), COL4A6(1288)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

human collagen type IV

General description

The composition of the extracellular matrix of all vertebrates is dominated by a class of molecules known as collagens, each with a unique feature suited to its function and location. Type IV collagen is a major constituent of basement membranes. This molecule is a continuous triple helix, about 400 nm in length, composed of at least two different αchain subunits termed α1 (IV) and α2 (IV) which may be composed of the same or different molecules. Upon proteolysis, fragments of 125 kDa, 95 kDa, and 60 kDa are released from a parent α1 (IV) chain, whereas fragments of 115 kDa, 80 kDa, and 45 kDa apparently originate from a distinct α2 (IV) chain. Collagens are known to have multiple functions. They are involved in tissue differentiation, morphogenesis and repair; play a role in the maintenance of tissue architecture; and form the filtration barrier of blood vessels.

Specificity

Using immunohistochemical staining of basal laminae, the antibody recognizes an epitope located on the α1 and/or α2 chains of human collagen type IV. The antibody shows reactivity with native primate collagen type IV in ELISA and dot blot assay. Using the dot blot technique, the antibody shows no cross-reactivity with human collagen types I, II, III, V, VI and VII, human vitronectin, fibronectin and chondroitin sulfate types A, B and C.

Application

Collagen, Type IV monoclonal antibody may be used in immunoassays such as immunocytochemistry, dot blot and ELISA. The antibody has been used for immunogold staining for electron microscopy, to visualize type IV collagen, in patients with coeliac disease. A minimum working dilution of 1:500 is determined by immunoperoxidase staining of formalin-fixed, paraffinembedded and enzyme-treated human tissue.

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