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C1985 Sigma

Anti-Clathrin Light Chain antibody, Mouse monoclonal

clone CON.1, purified from hybridoma cell culture



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell Membrane, Antibodies to Cell and Organelle Proteins,
species reactivity   mouse, rat, yeast, bovine, human
application(s)   immunocytochemistry: suitable
  immunoprecipitation: suitable
  microarray: suitable
  western blot: 1-2 μg/mL using whole rat brain homogenate
clone   CON.1, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2b
mol wt   antigen mol wt 25-36 kDa (doublet)
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CLTA(1211), CLTB(1212)
mouse ... Clta(12757), Cltb(74325)
rat ... Clta(83800), Cltb(116561)
biological source   mouse
conjugate   unconjugated



synthetic peptide (amino acids 23-44 in bovine) common to the N-terminus of mammalian clathrin light chains, LCa and LCb, conjugated to KLH. This region is not involved in heavy chain binding and is completely conserved in all mammals.

General description

Clathrin, light chain A also known as LCA is a soluble protein composed of heavy and light chain. Clathrin is a protein that plays a major role in the formation of coated vesicles. It coats vesicles from brain, adrenal medulla and non-secreting lymphoma cell line. Clathrin-coated vesicles (CCVs) are major carriers for endocytic cargo and maintain important intracellular trafficking events.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Monoclonal Anti-Clathrin Light Chain antibody produced in mouse is suitable for the following applications:
• Immunocytochemistry
• Immunoprecipitation
• Microarrary
• Western blotting at a concentration of 1-2μg/mL using whole rat brain homogenate

Biochem/physiol Actions

Clathrin requires cofactors like ADP-ribosylation factor 6 (ARF6) and adaptin for regulation of receptors. Clathrin light chains (CLCs) are not required for clathrin-mediated endocytosis but plays a critical role for clathrin-mediated trafficking between the TGN and the endosomal system whereas, clathrin heavy chain provides the structural backbone of the clathrin coat. CLCs in mammalian cells function in intracellular membrane trafficking by acting as recruitment proteins for huntingtin-interacting protein 1-related (HIP1R), enabling it to regulate actin assembly on clathrin-coated structures.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  nwg
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