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  • C1999 - CMP-Sialic Acid Synthetase from Neisseria meningitidis group B


C1999 Sigma

CMP-Sialic Acid Synthetase from Neisseria meningitidis group B

recombinant, expressed in E. coli BL21, ≥10 units/mg protein

Synonym: CTP: N-Acylneuraminate cytidylyltransferase



Analysis Note

Enzymatic activity assays are performed in Tris-HCl buffer (100 mM, pH 8.5) containing Neu-5-Ac (1 mM) and CTP (1 mM) at 37 °C for 30 min and analyzed using capillary electrophoresis with a UV detector (200 nm).

Unit Definition

One unit will catalyze the formation of 1 μmol CMP-Neu-5-Ac from Neu-5-Ac and CTP per minute at 37 °C at pH 8.0.

Physical form

Supplied as a lyophilized powder containing Tris-HCl and NaCl.


The enzyme has been utilized to synthesize CMP-sialic acid and its derivatives.1

Protocols & Applications

Sialic Acid Synthesis and Signaling Overview

Price and Availability

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Safety & Documentation

Safety Information

WGK Germany 

Protocols & Articles

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Peer-Reviewed Papers


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1. Chemoenzymatic synthesis of CMPsialic acid derivatives by a one-pot two-enzyme system: comparison of substrate flexibility of three microbial CMP-sialic acid synthetases. Yu H, et al. Bioorg. Med. Chem. 12, 6427-6435, (2004)


Purification and characterization of the recombinant CMP-sialic acid synthetase from Neisseria meningitides. Gilbert, M., et al Biotechnol. Lett. 19, 417-420, (1997)

Complete sequencing and characterization of 21,243 full-length human cDNAs. Ota, T. Nat. Genet. 36, 40-5, (2004)


Glycomimicry: display of the GM3 sugar epitope on Escherichia coli and Salmonella enterica sv Typhimurium. Ilg K, Yavuz E, Maffioli C, et al. Glycobiology 20(10), 1289-97, (2010)


Genetic engineering of Escherichia coli for the economical production of sialylated oligosaccharides. Fierfort N and Samain E J. Biotechnol. 134(3-4), 261-5, (2008)


Initial characterization of the human central proteome. Burkard TR, Planyavsky M, Kaupe I, et al. BMC Syst. Biol. 5, 17, (2011)


Haploid genetic screens in human cells identify host factors used by pathogens. Carette JE, Guimaraes CP, Varadarajan M, et al. Science 326(5957), 1231-5, (2009)


Site-specific immobilization of CMP-sialic acid synthetase on magnetic nanoparticles and its use in the synthesis of CMP-sialic acid. Yu CC, Lin PC, and Lin CC Chem. Commun. (Camb.) (11), 1308-10, (2008)


Addition of neuA, the gene encoding N-acylneuraminate cytidylyl transferase, increases the discriminatory ability of the consensus sequence-based scheme for typing Legionella pneumophila serogroup 1 strains. Ratzow S, Gaia V, Helbig JH, et al. J. Clin. Microbiol. 45(6), 1965-8, (2007)


Sialome analysis of the cephalochordate Branchiostoma belcheri, a key organism for vertebrate evolution. Guérardel Y, Chang LY, Fujita A, et al. Glycobiology 22(4), 479-91, (2012)


Construction of a functional CMP-sialic acid biosynthesis pathway in Arabidopsis. Castilho A, Pabst M, Leonard R, et al. Plant Physiol. 147(1), 331-9, (2008)


Deficits in sialylation impair podocyte maturation. Weinhold B, Sellmeier M, Schaper W, et al. J. Am. Soc. Nephrol. 23(8), 1319-28, (2012)


Bacterial CMP-sialic acid synthetases: production, properties, and applications. Mizanur RM and Pohl NL Appl. Microbiol. Biotechnol. 80(5), 757-65, (2008)


Stable coexpression of two human sialylation enzymes in plant suspension-cultured tobacco cells. Kajiura H, Misaki R, Fujiyama K, et al. J. Biosci. Bioeng. 111(4), 471-7, (2011)


The full-ORF clone resource of the German cDNA Consortium. Bechtel S, Rosenfelder H, Duda A, et al. BMC Genomics 8, 399, (2007)


The rainbow trout CMP-sialic acid synthetase utilises a nuclear localization signal different from that identified in the mouse enzyme. Tiralongo J, Fujita A, Sato C, et al. Glycobiology 17(9), 945-54, (2007)


Pasteurella multocida CMP-sialic acid synthetase and mutants of Neisseria meningitidis CMP-sialic acid synthetase with improved substrate promiscuity. Li Y, Yu H, Cao H, et al. Appl. Microbiol. Biotechnol. 93(6), 2411-23, (2012)


Chemoenzymatic synthesis of CMP-N-acetyl-7-fluoro-7-deoxy-neuraminic acid. Hartlieb S, Günzel A, Gerardy-Schahn R, et al. Carbohydr. Res. 343(12), 2075-82, (2008)


Chemo-enzymatic synthesis of C-9 acetylated sialosides. Rauvolfova J, Venot A, and Boons GJ Carbohydr. Res. 343(10-11), 1605-11, (2008)


Chemoenzymatic synthesis of mono- and di-fluorinated Thomsen-Friedenreich (T) antigens and their sialylated derivatives. Yan J, Chen X, Wang F, et al. Org. Biomol. Chem. 11(5), 842-8, (2013)


Cloning and characterization of a heat-stable CMP-N-acylneuraminic acid synthetase from Clostridium thermocellum. Mizanur RM and Pohl NL Appl. Microbiol. Biotechnol. 76(4), 827-34, (2007)


Identification and biochemical characterization of two functional CMP-sialic acid synthetases in Danio rerio. Schaper W, Bentrop J, Ustinova J, et al. J. Biol. Chem. 287(16), 13239-48, (2012)


Efficient chemoenzymatic synthesis of sialyl Tn-antigens and derivatives. Ding L, Yu H, Lau K, et al. Chem. Commun. (Camb.) 47(30), 8691-3, (2011)


Parallel chemoenzymatic synthesis of sialosides containing a C5-diversified sialic acid. Cao H, Muthana S, Li Y, et al. Bioorg. Med. Chem. Lett. 19(20), 5869-71, (2009)


Identification and characterization of important residues in the catalytic mechanism of CMP-Neu5Ac synthetase from Neisseria meningitidis. Horsfall LE, Nelson A, Berry A. FEBS J. 277, 2779-2790, (2010)


Identification of the nuclear export signals that regulate the intracellular localization of the mouse CMP-sialic acid synthetase. Fujita A, Sato C, and Kitajima K Biochem. Biophys. Res. Commun. 355(1), 174-80, (2007)


Cloning and expression of human sialic acid pathway genes to generate CMP-sialic acids in insect cells. Lawrence SM, Huddleston KA, Tomiya N, et al. Glycoconj. J. 18(3), 205-13, (2001)


Synthesis of sulfone-based nucleotide isosteres: identification of CMP-sialic acid synthetase inhibitors. Wong JH, Sahni U, Li Y, et al. Org. Biomol. Chem. 7(1), 27-9, (2009)


A C-terminal phosphatase module conserved in vertebrate CMP-sialic acid synthetases provides a tetramerization interface for the physiologically active enzyme. Oschlies M, Dickmanns A, Haselhorst T, et al. J. Mol. Biol. 393(1), 83-97, (2009)


Direct detection of ligand binding to Sepharose-immobilised protein using saturation transfer double difference (STDD) NMR spectroscopy. Haselhorst T, Münster-Kühnel AK, Oschlies M, et al. Biochem. Biophys. Res. Commun. 359(4), 866-70, (2007)


A CMP-N-acetylneuraminic acid synthetase purified from a marine bacterium, Photobacterium leiognathi JT-SHIZ-145. Kajiwara H, Mine T, Miyazaki T, et al. Biosci. Biotechnol. Biochem. 75(1), 47-53, (2011)


The sialic acids. X. Purification and properties of cytidine 5'-monophosphosialic acid synthetase. Kean EL and Roseman S J. Biol. Chem. 241(23), 5643-50, (1966)


The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard, DS. Genome Res. 14, 2121-7, (2004)


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