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C2206 Sigma

Anti-β-Catenin antibody produced in rabbit

whole antiserum

Properties

Related Categories AA - CA, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Embryonic Stem Cells,
species reactivity   several mammalian species
application(s)   dot blot: suitable using the immunogen and related peptides conjugated to BSA
  immunocytochemistry: 1:2,000 using cultured MDBK cells
  immunohistochemistry: 1:2,000 using bovine kidney frozen sections
  western blot: 1:4,000 using cultured MDBK cells
clone   polyclonal
antibody form   whole antiserum
mol wt   antigen mol wt 88-94 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CTNNB1(1499)
mouse ... Ctnnb1(12387)
rat ... Ctnnb1(84353)
biological source   rabbit
conjugate   unconjugated
Featured Industry   Research Pathology

Description

Immunogen

synthetic peptide corresponding to the C-terminal region (amino acids 768-781) of human/mouse β-catenin, conjugated with gluteraldehyde to KLH.

General description

The distinct peripheral cytosolic proteins, α-, β- and gamma-catenin (102 kDa, 94 kDa and 86 kDa respectively), are found in varying abundance in many developing and adult tissues. The catenins bind, directly or indirectly, to the conserved cytoplasmic tail domain of the cell adhesion cadherins. Cadherins are transmembrane cell surface glycoprotein molecules, concentrated at adherens junctions that mediate calcium-dependent intercellular interactions and are important for tissue morphogenesis.
Plakoglobin (probably identical to gamma-catenin) and β-catenin are structural and possibly functional mammalian homologues of armadillo (arm), a Drosophila protein involved in signal transduction. The protein, β-catenin, binds directly to the cytoplasmic tail of Ecadherin. It binds to the amino terminus of α-catenin and also interacts with the cytosolic protein product of the human tumor suppressor gene APC. The central core region of β-catenin is involved in mediation of the interaction of cadherin-catenin complex with the epidermal growth factor receptor. The protein, β-catenin, is the target of two signal transduction pathways mediated by the protooncogenes Src and Wnt-1. p120cas which exhibits structural similarity to β-catenin and plakoglobin may represent another catenin associated with cadherin.

Specificity

The antibody does not cross react with α-catenin or γ-catenin (plakoglobin).

Application

Polyclonal antibodies against defined type-specific catenin peptides are useful tools for the study of these proteins. Such antibodies recognize the respective catenin type in a variety of immunological techniques such as immunoprecipitation, immunoblotting, immunofluorescence and immunoperoxidase in different cell types from various species.

Price and Availability


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