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C226 Sigma

Monoclonal Anti-Calcitonin Gene-Related Peptide antibody produced in mouse

clone C4/19, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-CGRP

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Properties

Related Categories Alphabetical Index, Antibodies, CA1-CAL, Calcitonin and Related Products, Cell Biology,
biological source   mouse
antibody form   purified immunoglobulin
clone   C4/19, monoclonal
form   buffered aqueous solution
species reactivity   rat, human
application(s)   dot blot: 1:10,000 using rat CGRP-BSA
  immunohistochemistry: 1:8,000 using 4% paraformaldehyde perfusin/rat brain
  radioimmunoassay: 1:5,000 using 125I-CGRP (rat)
isotype   IgG1
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

rat α-CGRP conjugated to BSA

Preparation Note

The antibody is purified using protein A agarose, 0.2 μm-filtered and supplied at approximately 1 mg/ml.

Specificity

The antibody reacts by ELISA with rat α- and β-CGRP and human α- and β-CGRP. By RIA the antibody has a KD = 1.9 nM. The antibody may be used to localize and detect calcitonin gene-related peptide (CGRP) -containing axon terminals in the cortex, caudate putamen and cell bodies, axons and terminals of CGRP-containing neurons in the CNS and periphery.

Physical form

Solution in 0.01 M phosphate buffered saline, containing 0.1% sodium azide

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
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