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  • C2493 - Anti-CENP-A (N-terminal) antibody produced in rabbit

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C2493 Sigma

Anti-CENP-A (N-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-CenH3, Anti-Centomeric protein A

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Epigenetics, Antibodies for Gene Regulation and Expression,
species reactivity   human, bovine (predicted)
application(s)   western blot: 0.5-1 μg/mL using HEK-293T cells expressing human CENP-A
clone   polyclonal
concentration   ~1.5 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 17 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CENPA(1058)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 4-17 located at the N-terminus of human CENP-A.

General description

Centromere protein A is a protein encoded by the CENPA gene in humans. It is a histone H3 variant that has centromere-specific nucleosomal property and is located at neocentromere found on chromosome 10. CENP-A- binding clusters is associated with nucleosomal blocks within the neocentromeric chromatin.

Physical form

Solution in 0.01 mM phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Application

Anti-CENP-A (N-terminal) antibody produced in rabbit is suitable for western blotting at a concentration of 0.5-1μg/mL using HEK-293T cells expressing human CENP-A.

Biochem/physiol Actions

The location of the centromere on the chromosome is dependent on the histone H3 variant centromere protein A (CENP-A). The N-terminal tail of CENP-A is highly different from other H3 variants. Serine 7 of CENP-A plays an important role in mitosis-specific phosphorylation. It also plays a key role in kinetochore assembly. Alteration in CENP-A causes various human cancers. CENP-A may play an important role in epithelial ovarian cancer (EOC) and may serve as prognostic marker. It acts as a potential target for gene therapy in the treatment of EOC. The NH2 terminus of human CENP-A is essential for mitotic progression and necessary for the platform function of CENP-A centromeric chromatin in the assembly as well as maintenance of active kinetochores.

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