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C2562 Sigma

Monoclonal Anti-Cytokeratin, pan (Mixture) antibody produced in mouse

clone C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2, ascites fluid

Synonym: Monoclonal Anti-Cytokeratin, pan (mixture)

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytokeratins,
species reactivity   wide range
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using protease-digested sections of human or animal tissues
  immunohistochemistry (frozen sections): suitable
  indirect immunofluorescence: 1:100 using protease-digested, formalin-fixed, paraffin-embedded sections of human or animal tissues
  western blot: suitable
clone   C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2, monoclonal
antibody form   ascites fluid
isotype   IgG1/IgG2a
contains   7% horse serum and 15 mM sodium azide as preservative
shipped in   dry ice
storage temp.   −20°C
biological source   mouse
conjugate   unconjugated

Description

Immunogen

mixture of several monoclonal cytokeratin clones.

Specificity

This is a mixture of monoclonal anti-cytokeratin antibodies. It recognizes human cytokeratins 1,4,5,6,8,10,13,18, and 19. It is a broad spectrum reagent, which reacts specifically with a wide variety of normal, reactive, and neoplastic epithelial tissues. The antibody mixture reacts with simple, cornifying and non-cornifying squamous epithelia and pseudostratified epithelia. It does not react with non-epithelial normal human tissues. Increased staining intensity is seen following proteolytic treatment (protease unmasking).

Application

Monoclonal Anti-Cytokeratin, pan (Mixture) antibody produced in mouse is suitable for the following applications:
• Immunohistochemistry (formalin-fixed, paraffin-embedded sections) using protease-digested sections of human or animal tissues.
• Immunohistochemistry (frozen sections).
• Indirect immunofluorescence (at a working dilution of 1:100 using protease-digested, formalin-fixed, paraffin-embedded sections of human or animal tissues).
• Immunocytochemical labeling (immunofluorescence ) of cells.
• Western blotting.

Biochem/physiol Actions

Cytokeratins are proteins of keratin-containing intermediate filaments that provide mechanical support and other additional functions in the epithelial cells. It is found in the intracytoplasmic cytoskeleton of epithelial tissue. Epithelial tissue expresses cytokeratin subunits in a specific and stable pattern. Cytokeratins along with vimentin are involved in cell proliferation, migration and differentiation of preodontoblasts and preameloblasts. The intermediate-sized filaments are abundant in human endothelial cells and are mostly of vimentin type.

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