Carbonic Anhydrase from bovine erythrocytes ≥95% (SDS-PAGE), specific activity ≥3,500 W-A units/mg protein, lyophilized powder

Properties

Related Categories	4.2.x.x C-O bonds, 4.x.x.x Lyases, Analytical and Industrial Enzymes, Application Index, Biochemicals and Reagents, Bovine Carbonic Anhydrase, Carbonic Anhydrase, Cell Signaling Enzymes, Enzyme Class Index, Enzymes, Inhibitors, and Substrates, General Metabolic Enzymes, General Metabolic Enzymes A-H, Metabolomics, Plasma & Blood Proteins, Plasma, Blood, and Related Proteins and Reagents, Proteins and Derivatives More...
assay	≥85% protein basis (biuret)
	≥95% (SDS-PAGE)
form	lyophilized powder
solubility	deionized water: >10 mg/mL
storage temp.	2-8°C

Description

Unit Definition

One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0°C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)

Application

Carbonic anhydrase, from bovine erythrocytes, is used to create carbon dioxide capture systems and to research various purification techniques ¹. Carbonic anhydrase is also used to study acid-base regulation in fish and carbonic anhydrase type II deficiency syndrome ^2,³.

Biochem/physiol Actions

Carbonic Anhydrase is a zinc-containing enzyme that catalyzes the reversible conversion of carbon dioxide to bicarbonate. One of its main physiological roles is to maintain the acid-base balance in blood and other tissues. Lack of carbonic anhydrase results in carbonic anhydrase type II deficiency syndrome, which is an autosomal recessive disease that causes osteopetrosis, renal tubular acidosis and brain calcifications ³.