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C2629 Sigma

Acetylcholinesterase from Electrophorus electricus (electric eel)

Type III, aqueous solution, ≥1,000 units/mg protein

Synonym: AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Biochemicals and Reagents, Cell Biology, Cell Signaling and Neuroscience,
type   Type III
form   aqueous solution
impurities   ~5 mg/mg protein (NH4)2SO4
color   clear to slightly hazy light yellow
shipped in   dry ice
storage temp.   −20°C

Description

Analysis Note

The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.

Biochem/physiol Actions

Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H2O to choline + acetic acid.

Caution

Excellent stability while frozen; avoid repeated freeze-thaw cycles.

Unit Definition

One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.

General description

Molecular Weight: 280 kDa
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)

Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.

Price and Availability

Safety & Documentation

Safety Information

WGK Germany 
3
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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