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C2687 Sigma

Monoclonal Anti-Calponin antibody produced in mouse

clone hCP, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Mesenchymal Stem Cells, Antibodies for Stem Cell Biology,
species reactivity   human, mouse, rabbit, pig, rat
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  immunoprecipitation: suitable
  microarray: suitable
  western blot: 1:10,000 using human uterus extract
clone   hCP, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 34 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CNN1(1264), CNN2(1265)
mouse ... Cnn1(12797), Cnn2(12798)
rat ... Cnn1(65204), Kcnip4(259243)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

human uterus smooth muscle extract.

Specificity

The antibody hCP (also cited as CALP), localizes calponin in mammalian smooth muscle. In human uterus, an additional band of approximately 27 kDa (l-calponin) may also be stained. The antibody does not cross-react with skeletal, cardiac or non-muscle tissue calponin. Nevertheless, the antibody exhibits cross-reactivity with an epitope (150 kDa range) in human or mouse skeletal muscle. In immunohistochemical staining, the product exhibits smooth muscle specificity. It stains vascular and visceral smooth muscle cells in tissue sections and primary cultured cells (or early passages), but not most cell lines originally derived from smooth muscle. The antibody does not stain epithelial, endothelial or connective tissue fibroblast cells. This product does not cross react with smooth muscle tissue from chicken.

Application

Monoclonal Anti-Calponin antibody produced in mouse is suitable for the following applications:
• Immunohistochemistry using formalin-fixed, paraffin-embedded sections
• Immunoprecipitation
• Microarray
• Western blotting (at a dilution of 1:10,000 using human uterus extract)
• Flow cytometry analysis

Biochem/physiol Actions

Calponin is a calcium binding protein that belongs to a family of actin-associated proteins. Calponin is necessary for autophosphorylation of protein kinase C (PKC) in vascular smooth muscle (VSM). Calponin-1 inhibition can prevent uterine smooth muscle cell migration, cause morphological change and rearrange F-actin without affecting its proliferation and apoptosis. Calponin h1 (CN) is a differentiation marker of smooth muscle cells and is down-regulated in the blood vessels of several human tumors. It can inhibit actomyosin ATPase activity. The h1 and h2 calponins bind F-actin and play a key role in regulating actin filaments in smooth muscle and non-muscle cells.

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