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  • C268 - Monoclonal Anti-m-Calpain (Domain III/IV) antibody produced in mouse

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C268 Sigma

Monoclonal Anti-m-Calpain (Domain III/IV) antibody produced in mouse

clone 107-82, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Intracellular Calcium Signaling, Antibodies to Calcium-Associated Proteins,
species reactivity   human, rat, pig, bovine
application(s)   indirect immunofluorescence: 1:50
  western blot: 1:500
clone   107-82, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 80 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CAPN2(824)
rat ... Capn2(29154)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

m-calpain from bovine skeletal muscle.

General description

Calpain-2 catalytic subunit is a protein encoded by the CAPN2 gene in humans. Calpains are calcium dependent proteases constituting a family of proteins that are involved in essential cellular functions mediated by calcium. They share a homologous cysteine-protease domain and an E-F hand Ca2+-binding domain. The calpain system consists of two ubiquitous forms of calpain (m-calpain and μ-calpain), a tissue specific calpain (n-calpain) and a calpain inhibitory protein (calpastatin).

Physical form

Solution containing 0.05% sodium azide.

Specificity

Epitope mapping studies indicate the epitope is between amino acids 502-699 (domain III/IV) of human m-calpain. The antibody reacts specifically with m-calpain. It does not cross-react with μ-calpain, n-calpain, calmodulin or calpastatin. It is not recommended for immunoprecipitation. By immunoblotting, reactivity is observed with human platelets and erythrocytes, bovine platelets, heart and skeletal muscle and with rat myoblasts, kidney, liver and spleen. By immunofluorescence on pig LLC-PK1 cells, diffuse cytoplasmic staining is observed.

Application

Monoclonal Anti-m-Calpain (Domain III/IV) antibody produced in mouse is suitable for indirect immunofluorescence at a dilution of 1:50 and for western blotting at a dilution of 1:500.

Biochem/physiol Actions

Calpain plays an important role during cellular processes including cytoskeletal remodeling, apoptosis and survival. Calpain-2 over expression is significantly associated with platinum resistant tumours and in ovarian carcinomas may allow prognostic stratification of patients treated with surgery and platinum-based chemotherapy. Its over expression in basal-like or triple-negative disease is associated with adverse breast cancer-specific survival. m-calpain (calpain-2) is significantly associated with triple-negative and basal-like disease.

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Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  nwg
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Peer-Reviewed Papers
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