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C270 Sigma

Monoclonal Anti-Calpastatin antibody produced in mouse

clone 1F7E3D10, ascites fluid, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Intracellular Calcium Signaling, Antibodies to Calcium-Associated Proteins,
species reactivity   pig, rat, bovine, human
application(s)   indirect immunofluorescence: 1:200
  western blot: 1:5,000
clone   1F7E3D10, monoclonal
antibody form   ascites fluid
form   buffered aqueous solution
isotype   IgG1
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CAST(831)
rat ... Cast(25403)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

calpastatin from bovine skeletal muscle.

General description

Calpastatin is a protein encoded by the CAST gene in humans. Calpastatin is an endogenous inhibitor of the ubiquitous calcium dependent proteinases μ- and m-calpain. It plays a key role in many cellular physiological and pathological processes. Calpastatin level is very important for controlling calpain activity.

Physical form

Solution in phosphate buffered saline and contains 0.05% sodium azide.

Specificity

Epitope mapping studies indicate the epitope is between amino acids 543-673 (domain IV) of human calpastatin. By immunoblotting, the antibody may detect bands at 150, 125, 90 and 70 kDa, which have been suggested to be proteins translated from different start sites of the calpastatin gene. It does not cross-react with calpains or calmodulin. By immunoblotting, reactivity is observed with human platelets and erythrocytes, bovine platelets, heart and skeletal muscle and in rat myoblasts, kidney, liver and spleen. By immunofluorescence on pig LLC-PK1 cells, diffuse cytoplasmic staining is observed.

Application

Monoclonal Anti-Calpastatin antibody produced in mouse is suitable for indirect immunofluorescence at a dilution of 1:200 and for western blotting at a dilution of 1:5,000.

Biochem/physiol Actions

Calpastatin availability in cytosol is controlled by Ca2+ and cyclic AMP. Prolonged calpain activation promotes degradation of calpastatin. Calpastatin is found to be overexpressed in mycoplasma-contaminated cultured cells. CAST gene is found to be involved in genetic susceptibility to Keratoconus (KC), a genetically heterogeneous corneal dystrophy. It is also found to be associated with Parkinson′s disease (PD) and single nucleotide polymorphisms (SNPs).

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