• USA Home
  • C2888 - Acetylcholinesterase from Electrophorus electricus (electric eel)

EMAIL THIS PAGE TO A FRIEND
C2888 Sigma

Acetylcholinesterase from Electrophorus electricus (electric eel)

Type V-S, lyophilized powder, ≥1,000 units/mg protein

Synonym: AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl

Purchase

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Analysis Note

The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.

Biochem/physiol Actions

Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H2O to choline + acetic acid.

Caution

For dilute solutions (<1 mg/ml), add 1 mg/ml BSA to stabilize. Solutions containing at least 1 mg protein/ml in dilute neutral phosphate buffer are stable at least 6 months refrigerated.

Packaging

500, 1000, 5000 units in glass bottle

Unit Definition

One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.

Physical form

Lyophilized powder containing Tris buffer salts

General description

Molecular Weight: 280 kDa
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)

Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
Are solutions of Product C2888, Acetylcholinesterase from Electrophorus electricus (electric eel), harmed by freezing?
Yes, solutions may be harmed by freezing. The data sheet for Product C2888 indicates that solutions stabilized by the addition of BSA are stable when kept refrigerated for up to six months. A customer has reported to us that they tried freezing a solution to which BSA was added; they found that the solution had a 40 percent loss of activity after four months in the freezer. So our best recommendation is that solutions be stabilized with BSA and kept refrigerated, not frozen.
Show more questions
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

Related Products

inhibitor

Product #

Description

Add to Cart

D7910 3,4-Dichloroisocoumarin, serine protease inhibitor
H5777 (±)-Huperzine A, synthetic, ≥98% (TLC)
A9013 1,5-Bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide
A3773 9-Amino-1,2,3,4-tetrahydroacridine hydrochloride hydrate, ≥99%

related product

Product #

Description

Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?