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C2900 Sigma

Chloramphenicol Acetyltransferase from Escherichia coli

lyophilized powder, 50,000-150,000 units/mg protein (Lowry)

Synonym: Acetyl-CoA: chloramphenicol 3-O-acetyltransferase, CAT

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Properties

Related Categories 2.3.x.x Acyltransferases, 2.x.x.x Transferases, Application Index, Biochemicals and Reagents, Cell Biology,
form   lyophilized powder
mol wt   mol wt 75 kDa (three identical subunits)
shipped in   dry ice
storage temp.   −20°C
Gene Information   Escherichia coli ... cat(2847485)

Description

Biochem/physiol Actions

The enzyme responsible for chloramphenicol resistance in bacteria. Catalyzes the conversion of acetyl-CoA + chloramphenicol to CoA + chloramphenicol 3-acetate.

Unit Definition

One unit will convert 1.0 nanomole of chloramphenicol and acetyl-CoA to chloramphenicol 3-acetate and CoA per min at pH 7.8 at 25 °C.

Physical form

Partially purified; contains Tris buffer salts.

General description

The enzyme has a molecular weight of approximately 78,000 Da and pH optimum of 7.8.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Enzymatic Assay of Chloramphenicol Acetyltransferase

To standardize a procedure for the determination of the enzymatic assay of Chloramphenicol Acetyltransferase.
Keywords: Enzyme activity, Enzymology, Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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