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C2969 Sigma

Monoclonal Anti-I-CAM-1 (CD54) antibody produced in mouse

clone 8.4A6, purified immunoglobulin

Synonym: Anti-I-CAM-1, Monoclonal Anti-CD54

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells,
species reactivity   monkey, human
application(s)   flow cytometry: suitable
  immunocytochemistry: suitable
  immunohistochemistry (frozen sections): suitable
  indirect ELISA: suitable
clone   8.4A6, monoclonal
antibody form   purified immunoglobulin
isotype   IgG1
mol wt   antigen mol wt 85-110 kDa
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... ICAM1(3383)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

TNF-α activated human endothelial cells.

Antigen Background

CD54 (I-CAM-1) is a glycoprotein involved in mediation of intercellular adhesion. CD54 is expressed by activated endothelial cells and is also detected on cells of many other lineages and in plasma.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4.

Specificity

The antibody recognizes the human CD54 (I-CAM-1) surface antigen by flow cytometry, ELISA, immunocytochemistry, and immunohistochemistry. It inhibits human leukocyte adhesion to activated endothelial cells and stimulates homotypic aggregation of SKW3 cells. The epitope recognized by the antibody is localized in domain D2 of the CD54 molecule.
5th Workshop: code no. S 100

Preparation Note

Product filtered through a 0.2 μm filter

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

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Peer-Reviewed Papers
15

References

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