Monoclonal Anti-Cathepsin L antibody produced in mouse clone 33/2, ascites fluid

Properties

Related Categories	3-3B, Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to Cathepsins and Granzymes, Antibodies to Protein Degradation, Antibodies to Proteolysis/Proteases, Antibodies to Tumor-associated Enzymes, Application Index, Biochemicals and Reagents, CAM-CB, Cancer Research, Cathepsin L, Cell Signaling Enzymes, Clone Index, Enzymes, Enzymes, Inhibitors, and Substrates, Lysosomal Proteases, Primary Antibodies, Proteasome, Calpain and Lysosomal Proteases More...
species reactivity	rat, mouse, human, mink
application(s)	immunohistochemistry (frozen sections): suitable
	indirect ELISA: suitable
	microarray: suitable
	western blot: 1:200 using whole cell extract of a cultured mink lung cell line
clone	33/2, monoclonal
antibody form	ascites fluid
isotype	IgG1
mol wt	antigen (human cathepsin L) mol wt 25 kDa
	antigen (human procathepsin L) mol wt 42 kDa
contains	15 mM sodium azide
shipped in	dry ice
storage temp.	−20°C
Gene Information	human ... CTSL1(1514) mouse ... Ctsl(13039) rat ... Ctsl1(25697)

Description

Immunogen

procathepsin L isolated from the human lung cancer cell line EPLC 32M1.

Specificity

Reacts specifically with native and denatured forms of human cathepsin L and procathepsin L. Recognizes an epitope within amino acid residues GYGFEST (265-271 in procathepsin L and 169-175 in the mature cathepsin L molecule). The antibody may be used for the suppression of the malignant growth of myeloma cells. Cross-reactivity is observed with rat cathepsin L (strong), mouse procathepsin L (weak) and mink cathepsin and procathepsin L. Does not cross-react with human cathepsin types B, D, H and S, procathepsins B and D, rat cathepsin B, and mouse procathepsins B and D.

Price and Availability

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Documents

Certificate of Analysis

Antibody Explorer

C2970 - Datasheet (29 KB)

C2970-blot (13 KB )

Compatible Secondary Abs

Safety Information

WGK Germany

3

Technical information & documentation associated with this product is available in the Safety & Documentation tab.

References

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Monoclonal antibodies against cathepsin L and procathepsin L of different species. Weber, E., et al. Hybridoma 16, 159, (1997)

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Cathepsin L is responsible for processing and activation of proheparanase through multiple cleavages of a linker segment. Abboud-Jarrous, G., et al. J. Biol. Chem. 283, 18167-18176, (2008)

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Divalent metal transporter 1 in the kidney proximal tubule is expressed in late endosomes/lysosomal membranes: implications for renal handling of protein-metal complexes. Abouhamed, M., et al. Am. J. Physiol. Renal Physiol. 290, F1525-F1533, (2006)

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Local Retention Versus Systemic Release Of Soluble VEGF Receptor-1 Are Mediated By Heparin-binding And Regulated By Heparanase. Sela, S., et al. Circ. Res. 108, 1063-70, (2011)

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