|Related Categories||3-3B, Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology,|
|species reactivity||rat, mouse, human, mink|
|application(s)||immunohistochemistry (frozen sections): suitable|
|indirect ELISA: suitable|
|western blot: 1:200 using whole cell extract of a cultured mink lung cell line|
|antibody form||ascites fluid|
|mol wt||antigen (human cathepsin L) mol wt 25 kDa|
|antigen (human procathepsin L) mol wt 42 kDa|
|contains||15 mM sodium azide|
|shipped in||dry ice|
human ... CTSL1(1514)|
mouse ... Ctsl(13039)
rat ... Ctsl1(25697)
procathepsin L isolated from the human lung cancer cell line EPLC 32M1.
Reacts specifically with native and denatured forms of human cathepsin L and procathepsin L. Recognizes an epitope within amino acid residues GYGFEST (265-271 in procathepsin L and 169-175 in the mature cathepsin L molecule). The antibody may be used for the suppression of the malignant growth of myeloma cells. Cross-reactivity is observed with rat cathepsin L (strong), mouse procathepsin L (weak) and mink cathepsin and procathepsin L. Does not cross-react with human cathepsin types B, D, H and S, procathepsins B and D, rat cathepsin B, and mouse procathepsins B and D.
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Keywords: Buffers, Cell biology, Epigenetics, Immunohistochemistry, Immunoprecipitation, Molecular biology, Neuroscience, Western blot
Divalent metal transporter 1 in the kidney proximal tubule is expressed in late endosomes/lysosomal membranes: implications for renal handling of protein-metal complexes. Abouhamed, M., et al. Am. J. Physiol. Renal Physiol. 290, F1525-F1533, (2006)
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