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C2970 Sigma

Monoclonal Anti-Cathepsin L antibody produced in mouse

clone 33/2, ascites fluid

Synonym: Anti-CATL, Anti-CTSL, Anti-MEP

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to Cathepsins and Granzymes,
species reactivity   rat, mouse, mink, human
application(s)   immunohistochemistry (frozen sections): suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 1:200 using whole cell extract of a cultured mink lung cell line
clone   33/2, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen (human cathepsin L) mol wt 25 kDa
  antigen (human procathepsin L) mol wt 42 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CTSL1(1514)
mouse ... Ctsl(13039)
rat ... Ctsl1(25697)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

procathepsin L isolated from the human lung cancer cell line EPLC 32M1.

General description

Cathepsins are lysosomal proteases that play an important role in the intracellular degradation of exogenous and endogenous proteins, activation of enzyme precursors, and tumor invasion and metastasis. They are normally localized in lysosomes of almost all mammalian cells, but under certain conditions they can be secreted from the cell.

Specificity

Reacts specifically with native and denatured forms of human cathepsin L and procathepsin L. Recognizes an epitope within amino acid residues GYGFEST (265-271 in procathepsin L and 169-175 in the mature cathepsin L molecule). The antibody may be used for the suppression of the malignant growth of myeloma cells. Cross-reactivity is observed with rat cathepsin L (strong), mouse procathepsin L (weak) and mink cathepsin and procathepsin L. Does not cross-react with human cathepsin types B, D, H and S, procathepsins B and D, rat cathepsin B, and mouse procathepsins B and D.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Monoclonal Anti-Cathepsin L is suitable for ELISA, immunoblotting, immunohistochemistry (frozen sections) and for the suppression of the malignant growth of myeloma cells.

Biochem/physiol Actions

Cathepsin L (EC 3.4.22.15) is responsible for most of the intralysosomal protein breakdown of normal cells. Monoclonal Anti-Cathepsin L (mouse IgG1 isotype) is derived from the 33/2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with procathepsin L isolated from the human lung cancer line EPLC 32 M1. Monoclonal Anti-Cathepsin L reacts specifically with the native and denatured forms of human cathepsin L (25 kDa) and procathepsin L (42 kDa).

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  nwg
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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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