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C2976 Sigma

Anti-Caspase 8 (GD-13) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to Caspases,
species reactivity   human
application(s)   microarray: suitable
  western blot: 1:1,000 using human Burkitt lymphoma Raji cell extract
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen (caspase 8a) mol wt 55 kDa
  antigen (caspase 8b) mol wt 54 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CASP8(841)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide of the human caspase 8a/MACHα1 precursor (amino acids 362-374 with N-terminal added lysine), conjugated to KLH.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

The sequence used as the immunogen corresponds to the C-terminal region of the enzyme p18 subunit and is identical in other human caspase 8 isoforms (MACHα2, MACHα3, MACHβ) and has limited homology (approx. 50%) to mouse caspase 8. By immunoblotting, the antibody reacts with caspase 8a and caspase 8b.

Application

Anti-Caspase 8 (GD-13) is suitable for microarray. It is also suitable for western blot at a concentration of 1:1,000 using human Burkitt lymphoma Raji cell extract.

Biochem/physiol Actions

Caspase 8 cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. It also cleaves ADPRT. Caspase 8 hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. It is also a target for cowpox virus CRMA death inhibitory protein. Anti-Caspase 8 (GD-13) recognizes human caspase 8a (55 kDa) and caspase 8b (54kDa) by immunoblotting. Staining of caspase 8 in immunoblotting is specifically inhibited with caspase 8 immunizing peptide (human, amino acids 362-374). Caspases play a crucial role in apoptosis in almost every cell type.

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