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C2978 Sigma

CelLytic M

Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Analysis Note

Use 125 μl CelLytic M for 106-107 of suspended cells. For adherent cells, use 500-1,000 μL for a 100 mm plate; 200-400 μL for a 35 mm plate.

Features and Benefits

• Efficient: Up to 50% more efficient than freeze thaw, sonication and other products
• Non-denaturing: Does not interfere in downstream applications such immunoprecipitation, kinase and phosphatase assays, reporter gene assays and gel shift assays
• Convenient: Ready-to-use reagent requires no scraping from culture plates
• Fast: Rapid cell lysis at room temperature.

General description

CelLytic M is a proprietary detergent solution designed for efficient whole-cell protein extraction from cultured mammalian cells. It enables efficient and rapid cell lysis and solubilization of proteins for both suspension and adherent cells. Lysates can be used in many downstream applications without removing the CelLytic M such as reporter gene assays, Western blots/immunoprecipitation, electrophoretic mobility shift assays, phosphatase assays and kinase asssays.

Quantity

CelLytic M reagent efficiency for protein extraction has been tested on, but not limited to, HeLa, CHO, COS, HL-60, Jurkat, A431, PC-12, and Bovine Aorta Endothelial Cells (BAEC).

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Price and Availability


Amplified Detection

Ultrafree Centrifugal Filter Units
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Frequently Asked Questions

Will CelLytic™ M, Product No. C2978, interfere with protein concentration assays such as Bradford, Lowry and BCA?
We have tested and found CelLytic™ M to be compatible for Bradford Assay and BCA assays.  For the Lowry method, there is a background issue that is problematic for low protein concentrations, but may be subtracted with high protein concentrations.
Will Product No. C2978, CelLytic™ M solubilize membrane proteins?
CelLytic™ M can solubilize membrane proteins as well as cytoplasmic proteins and the conditions are non-denaturing.  However, we cannot guarantee the solubilization of all membrane proteins. The following proteins were detected in the CelLytic™ M lysate when tested with these antibodies against membrane proteins: Placental alkaline phosphatase, CD45, Connexin and Caveolin.
Is there any denaturants in Product C2978, CelLytic™ M that can interfere with my downstream applications?
CelLytic™ M does not contain any primary amines; it is a buffered solution (pH 7.6) which has no denaturants except for a mild detergent.  The exact formulation is proprietary.  This product may be diluted or dialyzed if it is found to have interference in your particular assay.
Does Product C2978, CelLytic™ M contain any protease inhibitors?
No, CelLytic™ M does not contain protease inhibitors.  We recommend the addition of one of our protease inhibitor cocktails, such as Product No. P8340 for mammalian cells or Product No. P2714 for general use.
Can Product C2978 CelLytic™ M be used for adherent cells?
Yes, CelLytic™ M can be used for both adherent cells and cells in suspension.  Use 125 µl CelLytic™ M for 106-107 of suspended cells. For adherent cells, use 500-1,000 µl for a 100 mm plate; 200-400 µl for a 35 mm plate. Click here to view this and other information on the Product Bulletin for CelLytic™ M. 
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles
Peer-Reviewed Papers
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References

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