|Related Categories||Cell Lysis, Cell Lysis Products, Cell Lysis and Protein Extraction, Core Bioreagents, HIS Select Supporting Products and Reagents,|
|storage temp.||room temp|
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Use 125 μl CelLytic M for 106-107 of suspended cells. For adherent cells, use 500-1,000 μL for a 100 mm plate; 200-400 μL for a 35 mm plate.
• Efficient: Up to 50% more efficient than freeze thaw, sonication and other products
• Non-denaturing: Does not interfere in downstream applications such immunoprecipitation, kinase and phosphatase assays, reporter gene assays and gel shift assays
• Convenient: Ready-to-use reagent requires no scraping from culture plates
• Fast: Rapid cell lysis at room temperature.
CelLytic M is a proprietary detergent solution designed for efficient whole-cell protein extraction from cultured mammalian cells. It enables efficient and rapid cell lysis and solubilization of proteins for both suspension and adherent cells. Lysates can be used in many downstream applications without removing the CelLytic M such as reporter gene assays, Western blots/immunoprecipitation
CelLytic M reagent efficiency for protein extraction has been tested on, but not limited to, HeLa, CHO, COS, HL-60, Jurkat, A431, PC-12, and Bovine Aorta Endothelial Cells (BAEC).
CelLytic is a trademark of Sigma-Aldrich Co. LLC
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