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C3062 Sigma

Anti-Cholera Toxin antibody produced in rabbit

whole antiserum

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to G Proteins, Antibodies to G Proteins and Cyclic Nucleotides,
biological source   rabbit
antibody form   whole antiserum
clone   polyclonal
contains   15 mM sodium azide
application(s)   Ouchterlony double diffusion: 1:16
  dot blot: 1:20,000
  indirect ELISA: 1:8,000
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

toxin from Vibrio cholerae

Preparation Note

delipidized

Application

Rabbit polyclonal anti-Cholera Toxin is used to tag cholera toxin for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as ELISA, immunoblotting, and immunoprecipitation. It may be used in studies on basic structural and functional aspects of toxin-membrane interactions.

General description

Cholera toxin, the pathogenic agent of cholera, is made of two subunits, A (27 kDa) and B (12 kDa) assembled with the stoichiometry AB5. The B-subunit binds to specific receptors, the monosialogangliosides GM1, located in the membrane of intestinal epithelial cells. The A1 fragment of the A-subunit is translocated through the membrane of the host cell, where it catalyses the ADP-ribosylation of the Gsa regulatory component of the adenylate cyclase complex. The resulting increased level of cyclic AMP promotes a wide variety of actions, including the secretion of chloride ions in the case of intestinal epithelial cells.

The antiserum reacts versus Cholera toxin, but shows no reaction versus Staphylococcal enterotoxin A, Staphylococcal enterotoxin B and Pseudomonas exotoxin A (protein concentration: 50-500 ng/dot). The product has not been tested for neutralization potency against active Cholera toxin.

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