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C3104 Sigma

Anti-CD30 antibody produced in goat

affinity isolated antibody, lyophilized powder

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology,
species reactivity   mouse
application(s)   indirect ELISA: 0.5-1.0 μg/mL
  western blot: 0.1-0.2 μg/mL
clone   polyclonal
antibody form   affinity isolated antibody
form   lyophilized powder
storage temp.   −20°C
Gene Information   mouse ... Tnfrsf8(21941)
biological source   goat
conjugate   unconjugated

Description

Immunogen

recombinant mouse CD30, extracellular domain, expressed in NSO cells.

General description

CD30 is a type I transmembrane glycoprotein of the TNF receptor superfamily. The extracellular domain of recombinant mouse CD30/Fc is fused to the carboxyterminal 6X histidine-tagged Fc portion of human IgG1 by a polypeptide linker.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline

Specificity

Shows no cross-reactivity with recombinant human CD30. The antibody will stimulate the bioactivity of mouse splenic B cell proliferation in the presence of IL-4 and IL-5.

Application

Anti-CD30 is suitable for western blotting at a concentration of 0.1-0.2μg/mL and indirect ELISA at a concentration of 0.5-1.0μg/mL

Reconstitution

Reconstitute with 1 mL sterile PBS to yield 0.1 mg/ml solution.

Biochem/physiol Actions

Anti-CD30 recognizes recombinant mouse CD30 by immunoblotting and ELISA. In immunoblotting and ELISA, this antibody does not cross-react with recombinant human CD30. Reduced mouse CD30/Fc monomer has a calculated molecular mass of approximately 55 kDa. Due to glycosylation, the recombinant protein migrates as an approximately 90 -100 kDa protein in SDS-PAGE under reducing conditions. CD30 specific IgG is purified from goat serum using mouse CD30 affinity chromatography. CD30 has a critical role in the pathophysiology of Hodgkin′s disease and other CD30+ lymphomas. CD30 acts as a costimulatory molecule in thymic negative selection. Anti-CD30 is developed in goat using purified recombinant mouse CD30 extracellular domain, expressed in mouse NSO cells as immunogen.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  1
Protocols & Articles

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Peer-Reviewed Papers
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References

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