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C3142 Sigma

α-Chymotrypsin from bovine pancreas

(TLCK treated to inactivate residual tryspin activity), Type VII, essentially salt-free, lyophilized powder, ≥40 units/mg protein

Synonym: TLCK-Chymotrypsin

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Properties

Related Categories 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Dissociation,
type   Type VII
form   essentially salt-free, lyophilized powder
mol wt   mol wt 25 kDa
solubility   1 mM HCl: soluble 10 mg/mL clear
storage temp.   −20°C
Gene Information   cow ... CTRB1(618826)

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Analysis Note

Protein determined by A1%/280

Biochem/physiol Actions

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.

α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. Its pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, α2-macroglobulin, 10 mM Cu2+ and Hg2+.

Other Notes

View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer

Packaging

10, 25, 100 mg in glass bottle

Preparation Note

TLCK treatment inactivates trypsin which may be present in chymotrypsin, without affecting the chymotrypsin activity.

Unit Definition

One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

Application

α-Chymotrypsin from Sigma has been used to determine the crystal structures of two homologous inhibitors (pars intercerebralis major peptide-C and pars intercerebralis major peptide-D2v) from the insect Locusta migratoria by forming a complex with the enzyme.

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Laboratory Gloves

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
GC3050000

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What is the molecular weight of alpha-chymotrypsin from bovine pancreas (Product No. C3142)?
The molecular weight of alpha chymotrypsin, reported in the literature, is approximately 25 kDa. This information and the basic structure description of the enzyme can be found on our product information sheet at our website. Here is the link to that document: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/c3142pis.Par.0001.File.tmp/c3142pis.pdf.
How do you recommend preparing a solution of Product No. C3142?
It is recommended to reconstitute Product No. C3142 in 1 mM hydrochloric acid containing 2 mM calcium chloride. The calcium functions as both a stabilizer (and possibly an activator) of the enzyme. Please refer to our product information sheet: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/c7762pis.Par.0001.File.tmp/c3142pis.pdf.
How can solutions of alpha-chymotrypsin (Product No. C3142) be stored?
Stock solutions prepared in 1 mM HCl, containing 2 mM calcium chloride, can be stored at -20 ° for about one week.
What is the pH optimum for the enzyme, Product No. C3142?
The pH optimum for alpha-chymotrypsin is between pH 7.5-8.5. Please see the following reference: Wilcox, P.E., Chymotrypsingogens-chymotrypsins, Methods in Enzymology, 19, 64-80 (1970).
Show more questions
Protocols & Articles

Protocols

Procedure for Enzymatic Assay of Chymotrypsin (α‑Chymotrypsin, EC 3.4.21.1)

This procedure is for products with a specification for Chymotrypsin activity. It is not to be used to assay Insoluble Chymotrypsin such as Catalog No. C9134. The procedure is a continuous spectropho...
Keywords: Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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