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C3175 Sigma

Chloramphenicol-Water Soluble

powder, BioReagent, suitable for cell culture



Related Categories Antibiotic powders, Antibiotics, Antibiotics A to Z, Antibiotics A-F, Biochemicals and Reagents,
product line   BioReagent
form   powder
solubility   H2O: 50-500 mg/mL (stock solution)
  PBS: 50-500 mg/mL (stock solution)
  other salt solutions: not recommended
suitability   suitable for cell culture
Mode of action   protein synthesis | interferes
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria


Frequently Asked Questions

Frequently Asked Questions are available for this Product.


Recommended for use in cell culture applications at approximately 5 μg/ml of active component (chloramphenicol).

Biochem/physiol Actions

Mode of Action: Inhibits elongation of peptidyl transferase.

Other Notes

Formulated to contain approx. 100 mg chloramphenicol per gram of powder; balance 2-hydroxypropyl-β-cyclodextrin.

Keep container tightly closed in a dry and well-ventilated place. Light sensitive. Keep in a dry place.


100 mg in glass bottle

Package size based on chloramphenicol

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Price and Availability

All labs need water
Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
  How do I calculate the molarity of product C3175, Chloramphenicol-Water Soluble?
Product C3175 (Chloramphenicol-Water Soluble) is a complex of chloramphenicol and cyclodextrin. Cyclodextrin is used to aid in the solubility of chloramphenicol into water. In order to prepare a molar solution of chloramphenicol you will have to base it on the chloramphenicol content in the solution and not the entire complex.  Product C3175 is formulated to contain approximately 100 mg chloramphenicol per gram of powder.  Based on this average, if you are preparing a 500 mg/ml stock solution, there will be approximately 50 mg of chloramphenicol in 1 ml.  Below is the calculated molarity of chloramphenicol when the complex is dissolved at 500 mg/ml in water.  molecular weight X unknown molarity X volume = complex weight X complex ratio 323.13 g/mol X Molarity X 1 ml = 500 mg X 0.1           Molarity = 0.155 M chloramphenicol molecular weight = 323.13 g/mol molarity (unknown) = mol/L volume = 1ml complex weight = 500 mg complex ratio = 100 mg chloramphenicol/1 gm complex
  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
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Protocols & Articles

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