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C3260 Sigma

Citrate Synthase from porcine heart

ammonium sulfate suspension, ≥100 units/mg protein

Synonym: Citrate condensing enzyme, Citrate oxaloacetate lyase (CoA-acetylating)

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Properties

Related Categories 4.1.x.x C-C bonds, 4.x.x.x Lyases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
form   ammonium sulfate suspension
solubility   H2O: soluble1.0 mg/mL, clear
foreign activity   isocitrate dehydrogenase and aconitase ≤0.01%
  malic dehydrogenase ≤0.1%
storage temp.   2-8°C

Description

Preparation Note

Dissolves in water to form a clear solution at 1 mg/mL concentration.

Unit Definition

One unit will form 1.0 μmole of citrate from oxalacetate and acetyl CoA per min at pH 8.0 at 37 °C.

Physical form

Suspension in 3.2 M (NH4)2SO4 solution, pH 7.0.

Biochem/physiol Actions

Citrate synthase catalyses the conversion of citrate to acetyl-CoA in the presence of coenzyme-A with the release of H2O and oxaloacetate. The enzyme has a molecular weight of 85 kDa and a pI of 6.1-6.6. It is inhibited by fluoroacetyl-CoA, palmitoyl-CoA, and citroyl-CoA. It is also inhibited when it is acetylated by acetic anhydride or iodinated by iodine.

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All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

Articles

Ammonium Sulfate Suspensions - Technical Note

Note that for an ammonium sulfate suspension, most of the enzyme will be in solid form. It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution.
Keywords: Enzyme activity

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

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