Synonym: AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl
|Related Categories||3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Acetylcholinesterase (AChE), True, RBC or Erythrocyte Cholinesterase, E.C. 184.108.40.206, Analytical and Industrial Enzymes, Application Index,|
|composition||Protein, ≥60% biuret|
The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.
Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H2O to choline + acetic acid.
One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.
Lyophilized powder containing Tris buffer salts
Molecular Weight: 280 kDa
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)
Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.
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≥98%, BioReagent, suitable for determination of sulfhydryl groups
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≥98% (TLC), powder
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Certificate of Origin
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Keywords: Cell culture, Cell disruption, Cell signaling, Diagnostic, Digestions, Drug discovery, Functional genomics, Gene expression, Genomics, Metabolic Pathways, Molecular biology, Neuroscience, Proteomics
Electrochemical biosensing of methyl parathion pesticide based on acetylcholinesterase immobilized onto Au-polypyrrole interlaced network-like nanocomposite. Gong, J., et al. Biosens. Bioelectron. 24, 2285-8, (2009)
Acetylcholinesterase Biosensor Design Based On Carbon Nanotube-encapsulated Polypyrrole And Polyaniline Copolymer For Amperometric Detection Of Organophosphates. Du, D., et al. Biosens. Bioelectron. 25, 2503-8, (2010)
Catalytic-site conformational equilibrium in nerve-agent adducts of acetylcholinesterase: possible implications for the HI-6 antidote substrate specificity. Artursson E, Andersson PO, Akfur C, et al. Biochem. Pharmacol. 85(9), 1389-97, (2013)
Dissecting the mechanisms responsible for the multiple insecticide resistance phenotype in Anopheles gambiae s.s., M form, from Vallée du Kou, Burkina Faso. Kwiatkowska RM, Platt N, Poupardin R, et al. Gene 519(1), 98-106, (2013)
Comparison of biochemical and molecular tests for detecting insecticide resistance due to insensitive acetylcholinesterase in Culex quinquefasciatus. Scott ML and McAllister JC J. Am. Mosq. Control Assoc. 28(4), 323-6, (2012)
Cholinergic signaling in the hippocampus regulates social stress resilience and anxiety- and depression-like behavior. Mineur YS, Obayemi A, Wigestrand MB, et al. Proc. Natl. Acad. Sci. U. S. A. 110(9), 3573-8, (2013)
7-Methoxytacrine-adamantylamine heterodimers as cholinesterase inhibitors in Alzheimer's disease treatment--synthesis, biological evaluation and molecular modeling studies. Spilovska K, Korabecny J, Kral J, et al. Molecules 18(2), 2397-418, (2013)
Insecticide resistance allele frequencies in Anopheles gambiae before and after anti-vector interventions in continental Equatorial Guinea. Reddy MR, Godoy A, Dion K, et al. Am. J. Trop. Med. Hyg. 88(5), 897-907, (2013)
Synthesis, characterization and biological screening of 5-substituted-1,3,4-oxadiazole-2yl-N-(2-methoxy-5-chlorophenyl)-2-sulfanyl acetamide. Rehman A, Fatima A, Abbas N, et al. Pak. J. Pharm. Sci. 26(2), 345-52, (2013)
Discovering and targeting the basic mechanism of neurodegeneration: the role of peptides from the C-terminus of acetylcholinesterase: non-hydrolytic effects of ache: the actions of peptides derived from the C-terminal and their relevance to neurodegeneration. Greenfield S Chem. Biol. Interact. 203(3), 543-6, (2013)
Acetylcholinesterase inhibition promotes retinal vasoprotection and increases ocular blood flow in experimental glaucoma. Almasieh M, MacIntyre JN, Pouliot M, et al. Invest. Ophthalmol. Vis. Sci. 54(5), 3171-83, (2013)
[Screening of endophytic fungi from Huperzia serrata for acetylcholinesterase inhibitory activity and its taxonomic identification]. Wang LL, Lv HF, Zhang L, et al. Zhongguo Zhong Yao Za Zhi 37(24), 3701-5, (2012)
Specific inhibitory effects of the alkylammonium derivative 6-methyluracil on acetylcholinesterase of smooth and striated muscles in rats. Nikitashina AD, Petrov KA, Zobov VV, et al. Dokl. Biol. Sci. 449, 82-4, (2013)
Purification of human red cell acetylcholinesterase by electrophoresis, ultracentrifugation and gradient extraction. ZITTLE CA, DELLAMONICA ES, CUSTER JH, et al. Arch. Biochem. Biophys. 56(2), 469-75, (1955)
Expression of three alternative acetylcholinesterase messenger RNAs in human tumor cell lines of different tissue origins. Karpel R, Ben Aziz-Aloya R, Sternfeld M, et al. Exp. Cell Res. 210(2), 268-77, (1994)
The effect of elimination of intersubunit disulfide bonds on the activity, assembly, and secretion of recombinant human acetylcholinesterase. Expression of acetylcholinesterase Cys-580----Ala mutant. Velan B, Grosfeld H, Kronman C, et al. J. Biol. Chem. 266(35), 23977-84, (1991)
Comparative analysis of the gene-dense ACHE/TFR2 region on human chromosome 7q22 with the orthologous region on mouse chromosome 5. Wilson MD, Riemer C, Martindale DW, et al. Nucleic Acids Res. 29(6), 1352-65, (2001)
Structures of recombinant native and E202Q mutant human acetylcholinesterase complexed with the snake-venom toxin fasciculin-II. Kryger G, Harel M, Giles K, et al. Acta Crystallogr. D, Biol. Crystallogr. 56(Pt 11), 1385-94, (2000)
Substrate binding to the peripheral site of acetylcholinesterase initiates enzymatic catalysis. Substrate inhibition arises as a secondary effect. Szegletes, T., et al. Biochemistry 38, 122-133, (1999)
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|H5777||(±)-Huperzine A, from synthetic, ≥98% (TLC)|
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