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C3515 Sigma

Catalase from Aspergillus niger

ammonium sulfate suspension, ≥4,000 units/mg protein

Synonym: H2O2:H2O2 oxidoreductase

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Description

Analysis Note

Protein determined by biuret

Caution

Freezing of solutions is not recommended.

Packaging

10, 25 mg in glass bottle

Unit Definition

One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.

Physical form

Suspension in 3.2 M (NH4)2SO4 solution, pH 6.0

Application

Catalase from Sigma has been used as a positive control during the functional characterization of Clostridium difficile spore coat proteins.

Biochem/physiol Actions

Catalase catalyzes the decomposition of hydrogen peroxide into water and oxygen. Each subunit of the tetramer contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group. Isoelectric point is found to be 6.5. Optimum pH for catalytic activity is 7.0. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu2+ results in degradation of the catalase molecule.

Price and Availability


Laboratory Gloves

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Ammonium Sulfate Suspensions - Technical Note

Note that for an ammonium sulfate suspension, most of the enzyme will be in solid form. It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution.
Keywords: Enzyme activity

HPLC Analysis of Proteins (2) on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins (2) on BIOshell™ A400 Protein C4
Keywords: Chromatography, High performance liquid chromatography, Reversed-phase chromatography

HPLC Analysis of Proteins on BIOshell™ A400 Protein C18, Hydrophobicity Comparision

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on BIOshell™ A400 Protein C18, Hydrophobicity Comparision
Keywords: High performance liquid chromatography

Protocols

Enzymatic Assay of Catalase (EC 1.11.1.6)

The continuous spectrophotometric rate reduction determination (A240, Light path = 1 cm) is based on the following reaction:
Keywords: Reductions

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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