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C3545 Sigma

Monoclonal Anti-Calmodulin antibody produced in mouse

clone 6D4, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Intracellular Calcium Signaling, Antibodies to Calcium-Associated Proteins,
species reactivity   bovine, rat, Dictyostelium discoideum, chicken
application(s)   western blot: 1:2,000 using a bovine brain calmodulin preparation
clone   6D4, monoclonal
antibody form   ascites fluid
isotype   IgG1
contains   15 mM sodium azide as preservative
shipped in   dry ice
storage temp.   −20°C
Gene Information   rat ... Calm1(24242)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

preparation of purified calmodulin from Dictyostelium discoideum conjugated to KLH.

General description

Calmodulin is a highly conserved 17kDa calcium binding protein found in all eukaryotic cells. It is a multifunctional, ubiquitous molecule which can bind up to four calcium ions, regulating and mediating a wide variety of biochemical processes. Monoclonal anti-Calmodulin (mouse IgG1 isotype) is derived from the 6D4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with calmodulin, purified from Dictyostelium discoideum and conjugated to KLH.

Specificity

The antibody recognizes native and SDS-denatured calmodulin.

These antibodies are directed against different epitopes within the calmodulin molecule.

Application

Monoclonal Anti-Calmodulin antibody produced in mouse is suitable for western blotting at a dilution of 1:2,000 using a bovine brain calmodulin preparation.

Biochem/physiol Actions

Monoclonal anti-Calmodulin, clone 6D4, recognizes an epitope found in SDS-denatured and native calmodulin, of the eukaryotic microorganism Dictyostelium discoideum and bovine, rat, and chicken brain, applying the immunoblotting technique

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