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C3678 Sigma

Anti-Pan Cadherin antibody produced in rabbit

whole antiserum

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Adhesion, Attachment, and Matrix Factors, Antibodies against Adhesion/Attachment/Matrix Factors, Antibodies for Cell Biology,
species reactivity   cat, rabbit, human, chicken
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using protease-digested animal heart sections
  indirect immunofluorescence: 1:100 using cultured MDBK cells
  western blot: 1:200
clone   polyclonal
antibody form   whole antiserum
mol wt   antigen mol wt 135 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   chicken ... CDH2(414745)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

Synthetic peptide corresponding to the C-terminal amino acids of chicken N-Cadherin with an extra N-terminal lysine residue (24 amino acids) coupled with glutaraldehyde to keyhole limpet hemocyanin (KLH).

General description

Cadherins are glycoprotein receptors that regulate Ca2+-dependant adhesion in cells. Cadherins are associated with adherens junctions and are mainly involved in morphogenesis of cells and tissues. P-cadherin is expressed in epithelial cells and its over-expression has been associated with breast cancer metastasis and invasion . Anti-Pan Cadherin antibody is specific for cadherins in a wide range of organisms including chickens, rabbits and rats.

Specificity

The epitope recognized by the antibody is shared by a wide variety of organisms.

Application

Anti-Pan Cadherin antibody is suitable for use in confocal immunofluorescence using rat ventricular myocytes and western blot (1:200). The antibody may also be used for indirect immunofluorescence (1:100 using cultured MDBK cells) and immunohistochemistry (in formalin-fixed, paraffin-embedded sections or at 1:1,000 dilutions using protease-digested animal heart sections). Additionally, the product can be used in indirect immunoblot (135 kDa band in chicken or rabbit heart extracts), immunoperoxidase and immunoelectron-microscopy assays.

May be used for the detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, and for demonstration of adherens type cell-cell junctions regardless of their cadherin type.

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