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C3867 Sigma

Collagen, Type I solution from rat tail

BioReagent, suitable for cell culture, sterile-filtered

Synonym: Collagen from rat tail

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Properties

Related Categories 2D Cell Culture, Adhesion, Attachment, and Matrix Factors, Adhesion, Attachment, and Matrix Factors for Stem Cell Expansion, Attachment Factors, Attachments Factors,
description   Approx. 100mg/vial (Volume 22 - 25ml/vial, see label for lot-specific protein concentration)
sterility   sterile-filtered
product line   BioReagent
surface coverage   6‑10 μg/cm2
suitability   suitable for cell culture
storage temp.   2-8°C
Gene Information   rat ... Col1a1(29393)

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Used as a coating material to support adherent cells growth and differentiation.

Other Notes

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Preparation Note

Recommended for use as a cell culture substratum. May not be suitable for 3-D gel formation.

Sterile solution prepared from rat tail tendons by a modification of the published extraction method 1. It is supplied as an aqueous solution in 20mM acetic acid with a protein concentration as stated on the bottle. (approx.100mg protein per vial). Protein concentration was estimated by the Biuret method 2.
SDS polyacrylamide gel electrophoresis analysis shows the typical band pattern for Type I collagen, with a doublet at apparent molecular weights of 115 and 130kDa and another doublet at 215 and 235kDa. Based on this analysis, the purity of the collagen sample is >95%.

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Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
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