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C3934 Sigma

Carbonic Anhydrase from bovine erythrocytes

lyophilized powder, ≥2,000 W-A units/mg protein

Synonym: Carbonate Dehydratase, Carbonate Hydrolyase

Purchase

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

100, 500 mg in poly bottle

Unit Definition

One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0°C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)

Application

Carbonic anhydrase from bovine erythrocytes (BCA) has been used to study the effect of removal of enzyme-bound metal ion, Zn2+, on aggregation behavior of the enzyme. Removal of metal ion by a chelator such as EDTA enhances the propensity of the enzyme to adopt the molten-globule state. This state of the enzyme is found to bind to the chaperone-like α-crystallin and prevent aggregation. The enzyme from Sigma has been immobilized to electrochemical transducers in order to obtain develop an analytical device for dissolved CO2 measurement. It has also been used for the thermodynamic analysis of conformational changes in BCA.

Biochem/physiol Actions

Carbonic anhydrase is a zinc metalloenzyme that has a molecular weight of approximately 30,000 Da. The enzyme catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in vital physiological and pathological processes such as pH and CO2 homeostasis, transport of bicarbonate and CO2, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. Therefore, this enzyme is an important target for inhibitors with clinical applications for various pathologies such as glaucoma, epilepsy and Parkinson’s disease.

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
What is the molecular weight of Product C3934, Carbonate Dehydratase?
Because there are a number of closely related isoforms, the molecular weight typically used is approximately 30 kDa.  This product is expected to be a mixture of isoforms.
What is the purity of Product C3934, Carbonate Dehydratase?
Since C3934 is a mixture of isoforms, no purity claim is made.  The product is packaged and offered based on its enzymatic activity.
Can stock solutions of Product C3934, Carbonate Dehydratase, be made and stored?
Carbonic anhydrase solutions can be prepared in water or buffer at concentrations up to 10 mg/mL.  It is common for there to be some particulates or haziness in the solution.  No data on solution stability has been collected by Sigma-Aldrich, so we recommend solutions be prepared fresh for use.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

Articles

Cellular Derived Blood Proteins and Enzymes

Cell damage is induced by reactive oxygen species (ROS). ROS are either free radicals, reactive anions containing oxygen atoms, or molecules containing oxygen atoms that can either produce free radic...
BioFiles 2006, 1.5, 4.
Keywords: Aerobic, Alzheimer Disease, Bacterial conjugations, Biofiles, Cancer, Metabolism, Oxidations, Parkinson Disease, Reductions, Respiration

HPLC Analysis of Proteins (2) on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins (2) on BIOshell™ A400 Protein C4
Keywords: Chromatography, High performance liquid chromatography, Reversed-phase chromatography

HPLC Analysis of Proteins on BIOshell™ A160 Peptide C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on BIOshell™ A160 Peptide C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins on BIOshell™ A400 Protein C18, Hydrophobicity Comparision

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on BIOshell™ A400 Protein C18, Hydrophobicity Comparision
Keywords: High performance liquid chromatography

HPLC Analysis of Proteins on SRT® SEC-1000, 30 cm x 7.8 mm I.D., 5 μm particles

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on SRT® SEC-1000, 30 cm x 7.8 mm I.D., 5 μm particles
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Proteins on SRT® SEC-300, 30 cm x 7.8 mm I.D., 5 μm particles

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on SRT® SEC-300, 30 cm x 7.8 mm I.D., 5 μm particles
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

Protocols

Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)

To standardize a procedure for the enzymatic assay of Carbonic Anhydrase (EC 4.2.1.1) for Wilbur-Anderson Units.
Keywords: Hydration reaction, Phase transitions

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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