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C3956 Sigma

Anti-c-Myc antibody produced in rabbit

~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Properties

Related Categories c-myc, Alphabetical Index, Antibodies, Antibodies and Conjugates, Antibodies for Cell Biology,
species reactivity   human
application(s)   immunocytochemistry: 5-10 μg/mL
  immunoprecipitation: 1-2 μg using immunoprecipitates a c-Myc fusion protein from transfected mammalian cell lysates or bacterial extracts
  indirect immunofluorescence: 5-10 μg/mL using detects c-Myc fusion proteins in methanol-acetone fixed transiently transfected cells
  microarray: suitable
  western blot: 0.5-1 μg/mL using detects c-Myc fusion proteins in cell extracts from transfected cultures as well as bacterial lysates.
clone   polyclonal
concentration   ~0.5 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
storage temp.   −20°C
Gene Information   human ... MYC(4609)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

peptide corresponding amino acids 408-425 of the human c-myc proto-oncogene, conjugated to maleimide-activated KLH through a C-terminal added cysteine residue.

General description

Anti-c-Myc antibody is used for the detection of the c-Myc tag sequence on c-Myc tagged fusion proteins.This polyclonal antibody is produced in rabbit and recognizes the c-Myc sequence at the N-terminus and C-terminus. C3956 is an Affinity purified antibody, increasing sensitivity in most applications.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Specificity

N-terminal or C-terminal c-Myc (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) tagged fusion proteins.

Application

Antibody is recommended for use in immunoblotting, immunoprecipitation and immunofluorescence.
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